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Characterization of phosphorus-regulated miR399 and miR827 and their isomirs in barley under phosphorus-sufficient and phosphorus-deficient conditions
dc.contributor.author | Hackenberg , Michael | |
dc.contributor.author | Shi, Bu-Jun | |
dc.contributor.author | Gustafson, Perry | |
dc.contributor.author | Langridge, Peter | |
dc.date.accessioned | 2014-06-25T10:47:41Z | |
dc.date.available | 2014-06-25T10:47:41Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Hackenberg, M.; et al. Characterization of phosphorus-regulated miR399 and miR827 and their isomirs in barley under phosphorus-sufficient and phosphorus-deficient conditions. BMC Plan Biology, 13: 214 (2013). [http://hdl.handle.net/10481/32362] | es_ES |
dc.identifier.issn | 1471-229 | |
dc.identifier.uri | http://hdl.handle.net/10481/32362 | |
dc.description.abstract | Background miR399 and miR827 are both involved in conserved phosphorus (P) deficiency signalling pathways. miR399 targets the PHO2 gene encoding E2 enzyme that negatively regulates phosphate uptake and root-to-shoot allocation, while miR827 targets SPX-domain-containing genes that negatively regulate other P-responsive genes. However, the response of miR399 and miR827 to P conditions in barley has not been investigated. | es_ES |
dc.description.abstract | Results In this study, we investigated the expression profiles of miR399 and miR827 in barley (Hordeum vulagre L.) under P-deficient and P-sufficient conditions. We identified 10 members of the miR399 family and one miR827 gene in barley, all of which were significantly up-regulated under deficient P. In addition, we found many isomirs of the miR399 family and miR827, most of which were also significantly up-regulated under deficient P. Several isomirs of miR399 members were found to be able to cleave their predicted targets in vivo. Surprisingly, a few small RNAs (sRNAs) derived from the single-stranded loops of the hairpin structures of MIR399b and MIR399e-1 were also found to be able to cleave their predicted targets in vivo. Many antisense sRNAs of miR399 and a few for miR827 were also detected, but they did not seem to be regulated by P. Intriguingly, the lowest expressed member, hvu-miR399k, had four-fold more antisense sRNAs than sense sRNAs, and furthermore under P sufficiency, the antisense sRNAs are more frequent than the sense sRNAs. We identified a potential regulatory network among miR399, its target HvPHO2 and target mimics HvIPS1 and HvIPS2 in barley under P-deficient and P-sufficient conditions. | es_ES |
dc.description.abstract | Conclusions Our data provide an important insight into the mechanistic regulation and function of miR399, miR827 and their isomirs in barley under different P conditions. | es_ES |
dc.language.iso | spa | es_ES |
dc.publisher | Biomed Central | es_ES |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | es_ES |
dc.subject | Barley | es_ES |
dc.subject | Phosphorus | es_ES |
dc.subject | miR399 | es_ES |
dc.subject | Isomir | es_ES |
dc.subject | Antisense | es_ES |
dc.subject | Targets | es_ES |
dc.subject | Differential expression | es_ES |
dc.title | Characterization of phosphorus-regulated miR399 and miR827 and their isomirs in barley under phosphorus-sufficient and phosphorus-deficient conditions | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.doi | 10.1186/1471-2229-13-214 |