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Delimiting the Origin of a B Chromosome by FISH Mapping, Chromosome Painting and DNA Sequence Analysis in Astyanax paranae (Teleostei, Characiformes)
dc.contributor.author | Silva, Duílio M. Z. de A. | |
dc.contributor.author | Pansonato-Alves, José Carlos | |
dc.contributor.author | Utsunomia, Ricardo | |
dc.contributor.author | Araya-Jaime, Cristian | |
dc.contributor.author | Ruiz-Ruano, Francisco J. | |
dc.contributor.author | Natal Daniel, Sandro | |
dc.contributor.author | Teruo Hashimoto, Diogo | |
dc.contributor.author | Oliveira, Cláudio | |
dc.contributor.author | Martínez Camacho, Juan Pedro | |
dc.contributor.author | Porto-Foresti, Fábio | |
dc.contributor.author | Foresti, Fausto | |
dc.date.accessioned | 2014-05-29T07:37:08Z | |
dc.date.available | 2014-05-29T07:37:08Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Silva, D.M.Z. de A.; et al. Delimiting the Origin of a B Chromosome by FISH Mapping, Chromosome Painting and DNA Sequence Analysis in Astyanax paranae (Teleostei, Characiformes). Plos One, 9(4): e94896 (2014). [http://hdl.handle.net/10481/32021] | es_ES |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/10481/32021 | |
dc.description.abstract | Supernumerary (B) chromosomes have been shown to contain a wide variety of repetitive sequences. For this reason, fluorescent in situ hybridisation (FISH) is a useful tool for ascertaining the origin of these genomic elements, especially when combined with painting from microdissected B chromosomes. In order to investigate the origin of B chromosomes in the fish species Astyanax paranae, these two approaches were used along with PCR amplification of specific DNA sequences obtained from the B chromosomes and its comparison with those residing in the A chromosomes. Remarkably, chromosome painting with the one-arm metacentric B chromosome probe showed hybridization signals on entire B chromosome, while FISH mapping revealed the presence of H1 histone and 18S rDNA genes symmetrically placed in both arms of the B chromosome. These results support the hypothesis that the B chromosome of A. paranae is an isochromosome. Additionally, the chromosome pairs Nos. 2 or 23 are considered the possible B chromosome ancestors since both contain syntenic H1 and 18S rRNA sequences. The analysis of DNA sequence fragments of the histone and rRNA genes obtained from the microdissected B chromosomes showed high similarity with those obtained from 0B individuals, which supports the intraspecific origin of B chromosomes in A. paranae. Finally, the population hereby analysed showed a female-biased B chromosome presence suggesting that B chromosomes in this species could influence sex determinism. | es_ES |
dc.description.sponsorship | This research was funded by grants from the State of São Paulo Research Foundation (FAPESP) to DMZAS (2011/16825-3) and CO (2010/17009-2), grants from National Council for Research and Development (CNPq) to FF and by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science (PLOS) | es_ES |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | es_ES |
dc.subject | Chromosome mapping | es_ES |
dc.subject | Chromosomes | es_ES |
dc.subject | DNA | es_ES |
dc.subject | DNA sequence analysis | es_ES |
dc.subject | Fungal genomics | es_ES |
dc.subject | Histones | es_ES |
dc.subject | Sex chromosomes | es_ES |
dc.title | Delimiting the Origin of a B Chromosome by FISH Mapping, Chromosome Painting and DNA Sequence Analysis in Astyanax paranae (Teleostei, Characiformes) | es_ES |
dc.type | journal article | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0094896 |