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dc.contributor.authorBali, Kiran Kumar
dc.contributor.authorHackenberg , Michael 
dc.contributor.authorLubin, Avigail
dc.contributor.authorKuner, Rohini
dc.contributor.authorDevor, Marshall
dc.date.accessioned2014-05-23T06:54:57Z
dc.date.available2014-05-23T06:54:57Z
dc.date.issued2014
dc.identifier.citationBali, K.K.; et al. Sources of individual variability: miRNAs that predispose to neuropathic pain identified using genome-wide sequencing. Molecular Pain, 10: 22 (2014). [http://hdl.handle.net/10481/31880]es_ES
dc.identifier.issn1744-8069
dc.identifier.urihttp://hdl.handle.net/10481/31880
dc.description.abstract[Background] We carried out a genome-wide study, using microRNA sequencing (miRNA-seq), aimed at identifying miRNAs in primary sensory neurons that are associated with neuropathic pain. Such scans usually yield long lists of transcripts regulated by nerve injury, but not necessarily related to pain. To overcome this we tried a novel search strategy: identification of transcripts regulated differentially by nerve injury in rat lines very similar except for a contrasting pain phenotype. Dorsal root ganglia (DRGs) L4 and 5 in the two lines were excised 3 days after spinal nerve ligation surgery (SNL) and small RNAs were extracted and sequenced. [Results] We identified 284 mature miRNA species expressed in rat DRGs, including several not previously reported, and 3340 unique small RNA sequences. Baseline expression of miRNA was nearly identical in the two rat lines, consistent with their shared genetic background. In both lines many miRNAs were nominally up- or down-regulated following SNL, but the change was similar across lines. Only 3 miRNAs that were expressed abundantly (rno-miR-30d-5p, rno-miR-125b-5p) or at moderate levels (rno-miR-379-5p) were differentially regulated. This makes them prime candidates as novel PNS determinants of neuropathic pain. The first two are known miRNA regulators of the expression of Tnf, Bdnf and Stat3, gene products intimately associated with neuropathic pain phenotype. A few non-miRNA, small noncoding RNAs (sncRNAs) were also differentially regulated. [Conclusions] Despite its genome-wide coverage, our search strategy yielded a remarkably short list of neuropathic pain-related miRNAs. As 2 of the 3 are validated regulators of important pro-nociceptive compounds, it is likely that they contribute to the orchestration of gene expression changes that determine individual variability in pain phenotype. Further research is required to determine whether some of the other known or predicted gene targets of these miRNAs, or of the differentially regulated non-miRNA sncRNAs, also contribute.es_ES
dc.description.sponsorshipThe study was supported by the German-Israel Foundation for Research and Development (GIF) and the Hebrew University Center for Research on Pain. R. K. and K.B. are members of the Molecular Medicine Partnership Unit, Heidelberg.es_ES
dc.language.isoenges_ES
dc.publisherBiomed Centrales_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 Licensees_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es_ES
dc.subjectDorsal root gangliones_ES
dc.subjectmiRNA-seqes_ES
dc.subjectNeuropathic paines_ES
dc.subjectRates_ES
dc.subjectRegulationes_ES
dc.subjectStrategyes_ES
dc.titleSources of individual variability: miRNAs that predispose to neuropathic pain identified using genome-wide sequencinges_ES
dc.typejournal articlees_ES
dc.rights.accessRightsopen accesses_ES
dc.identifier.doi10.1186/1744-8069-10-22


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