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dc.contributor.authorSerrato Ochoa, Deyanira
dc.contributor.authorNieto Aguilar, Renato
dc.contributor.authorGarzón Bello, Ingrid Johanna 
dc.contributor.authorRoda Murillo, Olga 
dc.contributor.authorCampos Muñoz, Antonio Jesús 
dc.contributor.authorAlaminos Mingorance, Miguel 
dc.date.accessioned2014-03-10T13:40:37Z
dc.date.available2014-03-10T13:40:37Z
dc.date.issued2009
dc.identifier.citationSerrato, D.; et al. Comparison of the effect of cryopreservation protocols on the histology of bioengineered tissues. Histology and Histophatology, 24(12): 1531-1540 (2009). [http://hdl.handle.net/10481/30772]es_ES
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10481/30772
dc.description.abstractThe purpose of this study was to compare the effects of five different cryopreservation protocols on the histology of bioengineered tissues. Although several artificial tissues have been developed to the date by tissue engineering, classical histological analysis methods and techniques must be optimized for these new tissues with special properties. The results of this study showed that the use of volatile solutions (formaldehyde, glutaraldehyde, glacial acetic acid and acetone) was not able to prevent the formation of large ice crystals that, in turn, can alter the structure of the artificial tissues. However, preincubation of the tissues in different concentrations of a carbon hydrate (glucose, maltose or trehalose) resulted in a better preservation of the tissue structure. We conclude that the best protocol that allows for an efficient analysis of the bioengineered tissues with very few artifacts is preincubation of the tissues in 0.300M or 0.400M trehalose for 30 or 120 min prior to OCT (optimal cutting temperature) embedding and cryosectioning. For all those reasons, we recommend the use of a cryoprotective agent before OCT embedding of human artificial tissues.es_ES
dc.description.sponsorshipThis work was supported by the grants FIS PI08/614 from the Spanish Instituto de Salud Carlos III and SAS PI-0132 from Junta de Andalucía.es_ES
dc.language.isoenges_ES
dc.publisherUniversidad de Murciaes_ES
dc.relation.urihttp://hdl.handle.net/10201/37553es_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 License
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subjectTissue engineeringes_ES
dc.subjectMethods es_ES
dc.subjectHistological analysises_ES
dc.subjectCryosectiones_ES
dc.subjectFibrin-agarose constructses_ES
dc.titleComparison of the effect of cryopreservation protocols on the histology of bioengineered tissueses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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