Mineralization of human premolar occlusal fissures: a quantitative histochemical microanalysis
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AutorCampos Muñoz, Antonio Jesús; Rodríguez, Ismael Ángel; Sánchez Quevedo, María Del Carmen; García López, José Manuel; Nieto Albano, O. H.; Gómez de Ferraris, María Elsa
Universidad de Murcia
Campos, A.; et al. Mineralization of human premolar occlusal fissures: a quantitative histochemical microanalysis. Histology and Histopathology, 15(2): 499-502 (2000). [http://hdl.handle.net/10481/30760]
PatrocinadorThis work was partially supported by the Ministerio de Educación y Cultura (PB97-0840) and the Agencia Española de Cooperación Internacional (AECI).
The mechanisms of cariogenesis in occlusal fissures remain elusive because of limited information about fissure structure and wall mineralization. The purpose of the present study was to determine the correlation between morphological patterns in occlusal fissures in human premolars and quantitative histochemical patterns of mineralization in the walls of these formations. We used scanning electron microscopy and quantitative X-ray microanalysis with the peak-tolocal background ratio method and microcrystalline calcium salts as standards. We distinguished three morphological patterns of fissures in scanning electron microscopic images. The wall of the fissures was less mineralized than the control enamel in all three types of fissures. Because the fissure walls are hypomineralized, we suggest that practicing dentists should take into account the degree of mineralization when they are preparing the fissures for the application of sealant.