Show simple item record

dc.contributor.authorGarzón Bello, Ingrid Johanna 
dc.contributor.authorCarriel Araya, Víctor 
dc.contributor.authorMarín Fernández, Ana Belén
dc.contributor.authorXimenes Oliveira, Ana Celeste
dc.contributor.authorGarrido-Gómez, Juan
dc.contributor.authorCampos Muñoz, Antonio Jesús 
dc.contributor.authorSánchez Quevedo, María Del Carmen 
dc.contributor.authorAlaminos Mingorance, Miguel
dc.identifier.citationGarzón, I.; et al. A Combined Approach for the Assessment of Cell Viability and Cell Functionality of Human Fibrochondrocytes for Use in Tissue Engineering. Plos One, 7(12): e51961 (2012). []es_ES
dc.identifier.otherdoi: 10.1371/journal.pone.0051961
dc.description.abstractTemporo-mandibular joint disc disorders are highly prevalent in adult populations. Autologous chondrocyte implantation is a well-established method for the treatment of several chondral defects. However, very few studies have been carried out using human fibrous chondrocytes from the temporo-mandibular joint (TMJ). One of the main drawbacks associated to chondrocyte cell culture is the possibility that chondrocyte cells kept in culture tend to de-differentiate and to lose cell viability under in in-vitro conditions. In this work, we have isolated human temporo-mandibular joint fibrochondrocytes (TMJF) from human disc and we have used a highly-sensitive technique to determine cell viability, cell proliferation and gene expression of nine consecutive cell passages to determine the most appropriate cell passage for use in tissue engineering and future clinical use. Our results revealed that the most potentially viable and functional cell passages were P5–P6, in which an adequate equilibrium between cell viability and the capability to synthesize all major extracellular matrix components exists. The combined action of pro-apoptotic (TRAF5, PHLDA1) and anti-apoptotic genes (SON, HTT, FAIM2) may explain the differential cell viability levels that we found in this study. These results suggest that TMJF should be used at P5–P6 for cell therapy protocols.es_ES
dc.description.sponsorshipThis work was supported by University of Granada-Campus de Excelencia Internacional, Subprograma de I+D+I y Transferencia and by the Spanish Ministry of Economy and Competitiveness, grant IPT-300000-2010-017 (INNPACTO program), co-financed by the European Regional Development Fund, European Union.es_ES
dc.publisherPublic Library of Science (PLOS)es_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 License
dc.subjectX-ray microanalysises_ES
dc.subjectTemporomandibular-joint disces_ES
dc.subjectElemental concentrationses_ES
dc.subjectArticular cartilagees_ES
dc.subjectEndothelial cellses_ES
dc.subjectStem cellses_ES
dc.titleA Combined Approach for the Assessment of Cell Viability and Cell Functionality of Human Fibrochondrocytes for Use in Tissue Engineeringes_ES

Files in this item


This item appears in the following Collection(s)

Show simple item record

Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Except where otherwise noted, this item's license is described as Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License