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dc.contributor.authorDelaye, Luis
dc.contributor.authorGonzález Domenech, Carmen M.
dc.contributor.authorGarcillán-Barcia, María P.
dc.contributor.authorPeretó, Juli
dc.contributor.authorCruz, Fernando de la
dc.contributor.authorMoya, Andrés
dc.date.accessioned2013-09-30T13:02:55Z
dc.date.available2013-09-30T13:02:55Z
dc.date.issued2011
dc.identifier.citationDelaye, L.; et al. Blueprint for a minimal photoautotrophic cell: conserved and variable genes in Synechococcus elongatus PCC 7942. BMC Genomics, 12: 25 (2011). [http://hdl.handle.net/10481/28224]es_ES
dc.identifier.issn1471-2164
dc.identifier.otherdoi: 10.1186/1471-2164-12-25
dc.identifier.otherPMID: 21226929
dc.identifier.otherPMCID: PMC3025956
dc.identifier.urihttp://hdl.handle.net/10481/28224
dc.description.abstractBackground: Simpler biological systems should be easier to understand and to engineer towards pre-defined goals. One way to achieve biological simplicity is through genome minimization. Here we looked for genomic islands in the fresh water cyanobacteria Synechococcus elongatus PCC 7942 (genome size 2.7 Mb) that could be used as targets for deletion. We also looked for conserved genes that might be essential for cell survival.es_ES
dc.description.abstractResults: By using a combination of methods we identified 170 xenologs, 136 ORFans and 1401 core genes in the genome of S. elongatus PCC 7942. These represent 6.5%, 5.2% and 53.6% of the annotated genes respectively. We considered that genes in genomic islands could be found if they showed a combination of: a) unusual G+C content; b) unusual phylogenetic similarity; and/or c) a small number of the highly iterated palindrome 1 (HIP1) motif plus an unusual codon usage. The origin of the largest genomic island by horizontal gene transfer (HGT) could be corroborated by lack of coverage among metagenomic sequences from a fresh water microbialite. Evidence is also presented that xenologous genes tend to cluster in operons. Interestingly, most genes coding for proteins with a diguanylate cyclase domain are predicted to be xenologs, suggesting a role for horizontal gene transfer in the evolution of Synechococcus sensory systems.es_ES
dc.description.abstractConclusions: Our estimates of genomic islands in PCC 7942 are larger than those predicted by other published methods like SIGI-HMM. Our results set a guide to non-essential genes in S. elongatus PCC 7942 indicating a path towards the engineering of a model photoautotrophic bacterial cell.es_ES
dc.description.sponsorshipFinancial support was provided by grants BFU2009-12895-C02-01/BMC (Ministerio de Ciencia e Innovación, Spain), the European Community’s Seventh Framework Programme (FP7/2007-2013) under grant agreement number 212894 and Prometeo/2009/092 (Conselleria d’Educació, Generalitat Valenciana, Spain) to A. Moya. Work in the FdlC laboratory was supported by grants BFU2008-00995/BMC (Spanish Ministry of Education), RD06/0008/1012 (RETICS research network, Instituto de Salud Carlos III, Spanish Ministry of Health) and LSHM-CT- 2005_019023 (European VI Framework Program). Dr. González-Domenech was supported by grant from the University of Granada. LD, thanks to financial support from Facultad de Ciencias, Universidad Nacional Autónoma de México.es_ES
dc.language.isoenges_ES
dc.publisherBiomed Centrales_ES
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/212894es_ES
dc.subjectBacterial proteinses_ES
dc.subjectGene transferes_ES
dc.subjectGenomees_ES
dc.subjectGenomic islandses_ES
dc.subjectPhylogeny es_ES
dc.subjectSynechococcuses_ES
dc.titleBlueprint for a minimal photoautotrophic cell: conserved and variable genes in Synechococcus elongatus PCC 7942es_ES
dc.typejournal articlees_ES
dc.rights.accessRightsopen accesses_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1186/1471-2164-12-25es_ES


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