Radioactive immunocomplex formation in a double antibody ria.

Abstract

A model has been produced for the kinetic and equilibrium study of antigen-antibody reactions in the radioimmunoassay (RIA) of C-Peptide double antibody. The aim is (1) to characterise the influence of initial concentrations of labelled (M) and unlabelled (Q) antigen and that of the initial concentration of the antibody in solution (P), and (2) to study the binding of the immunocomplex (PM) to the second antibody (J) immobilised on a bead, and the replacement of M by Q in the PMJ immunocomplex. In order to study the effect of such variables, 44 experiments were conducted. The results are in line with the model proposed.

Se ha elaborado un modelo para el estudio de la cinética y equilibrio de las reacciones antígeno-anticuerpo implicadas en el radioinmunoanálisis (RIA) de doble anticuerpo correspondiente al Péptido C. Se pretende caracterizar la influencia de las concentraciones iniciales de antígeno marcado (M) y no marcado (Q) sobre la formación del inmunocomplejo (PM) al segundo anticuerpo (J) inmovilizado sobre una bola. Se realizan 30 experiencias para el estudio del efecto de las variables antes mencionadas. Los resultados obtenidos son concordantes con el modelo propuesto.