Actividad IN VIVO del aceite esencial de Origanum elongatum frente a larvas L3 de Anisakis pegreffii

Abstract

En este trabajo se aborda la posible actividad larvicida in vivo del aceite esencial de Origanum elongatum, recolectado en Marruecos, frente a las larvas L3 de Anisakis pegreffii, que parasitan distintas especies marinas. Los resultados obtenidos son demostrativos del interés potencial de este aceite esencial como preventivo de la infección por Anisakis, tras ingestión del pescado parasitado.

Anisakiasis is an emerging zoonotic disease caused by species of the genus Anisakis. In humans, this parasite is manifested by digestive symptoms. In the virtual absence of effective treatments against this infection, our working group has initiated a series of investigations aimed at finding natural products such as essential oils and their major components, which might be of interest in the treatment of the infectious form of these zoonoses. OBJECTIVE: Establishment of the possible in vivo activity of essential oil of O. elongatum, against L3 larva of Anisakis pegreffii. METHODOLOGY: For the study in vivo, parasites were isolated from the host Scomber japonicus (mackerel) and Trachurus trachurus (horse mackerel). The experimental animals (female Wistar rats) were infected with 6 Anisakis larva by gastric catheter, this technique was used also for the administration of O. elongatum (46.9 mg / 0.5 ml of olive oil), according to the following guidelines: infection and joint treatment and sacrifice at 4 hours. Parallel to this, a control test was performed, administering 0.5 ml olive oil together with six larvae of the parasite to a group of animals. The identification of the larvae was carried out using molecular techniques (PCR-RFLP). The identification of the main components of essential oil was performed by GC-MS.