Comparison of shor-term estrogenicity tests for identification of hormone-disrupting chemicals
Metadatos
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Andersen, Helle Raun; Andersson, Anna-Maria; Arnold, Steven F.; Autrup, Herman; Barfoed, Marianne; Beresford, Nicola; Bjerregaard, Poul; Christiansen, Lisette; Gissel, Birgitte; Hummel, René; Jørgensen, Eva B.; Korsgaard, Bodil; Guevel, Remy Le; Leffers, Henrik; McLachlan, John A.; Møller, Anette; Nielsen, Jesper Bo; Olea Serrano, Nicolás; Oles-Karasko, Anita; Pakdel, Farzad; Pedersen, Knud L.; Pérez, Pilar; Skakkebæk, Niels; Sonnenschein, Carlos; Soto, Ana M.; Sumpter, John P.; Thorpe, Susan; Grandjean, PhilippeEditorial
National Institute of Environmental Health Sciences
Materia
Estrogenic chemicals Estrogens Antiestrogens Estrogenicity tests Binding assay Yeast MCF-7 Vitellogenin
Fecha
1999Referencia bibliográfica
Andersen, H.R.; et al. Comparison of shor-term estrogenicity tests for identification of hormone-disrupting chemicals. Environmental Health Perspectives, 107(sup.1): 89-108 (1999). [http://hdl.handle.net/10481/24990]
Patrocinador
This study was supported by grants from the European Commission (Biomedicine and Health Research and Technological Programme, BMH4-CT96-03 14), the Danish Environmental Research Programme (96.01.015.16), and the Danish Medical Research Council (9401656).Resumen
The aim of this study was to compare results obtained by eight different short-term assays of estrogenlike actions of chemicals conducted in 10 different laboratories in five countries. Twenty chemicals were selected to represent direct-acting estrogens, compounds with estrogenic metabolites, estrogenic antagonists, and a known cytotoxic agent. Also included in the test panel were 17β-estradiol as a positive control and ethanol as solvent control. The test compounds were coded before distribution. Test methods included direct binding to the estrogen receptor (ER), proliferation of MCF-7 cells, transient reporter gene expression in MCF-7 cells, reporter gene expression in yeast strains stably transfected with the human ER and an estrogen-responsive reporter gene, and vitellogenin production in juvenile rainbow trout. 17β-Estradiol, 17α-ethynyl estradiol, and diethylstilbestrol induced a strong estrogenic response in all test systems. Colchicine caused cytotoxicity only. Bisphenol A induced an estrogenic response in all assays. The results obtained for the remaining test compounds—tamoxifen, ICI 182.780, testosterone, bisphenol A dimethacrylate, 4-n-octylphenol, 4-n-nonylphenol, nonylphenol dodecylethoxylate, butylbenzylphthalate, dibutylphthalate, methoxychlor, o,p′-DDT, p,p′-DDE, endosulfan, chlomequat chloride, and ethanol—varied among the assays. The results demonstrate that careful standardization is necessary to obtain a reasonable degree of reproducibility. Also, similar methods vary in their sensitivity to estrogenic compounds. Thus, short-term tests are useful for screening purposes, but the methods must be further validated by additional interlaboratory and interassay comparisons to document the reliability of the methods.