Biodegradation of olive washing wastewater pollutants by highly efficient phenol-degrading strains selected from adapted bacterial community
MetadataShow full item record
AuthorMaza-Márquez, Paula; Martínez-Toledo, María Victoria; González López, Jesús; Rodelas González, María Belén; Juárez-Jiménez, Belén; Fenice, Massimiliano
Olive washing wastewaterPCR-TGGE characterizationPhenol-degrading bacteriaBiological treatment
Maza-Márquez, P.; et al. Biodegradation of olive washing wastewater pollutants by highly efficient phenol-degrading strains selected from adapted bacterial community. International Biodeterioration and Biodegradation, 82: 192-198 (2013). [http://hdl.handle.net/10481/24759]
SponsorshipThis research was supported by European project Algatec (FP7/SME/2008/1/232331).
The bacterial community of an olive washing water (OWW) storage basin was characterized, by both cultivation and cultivation-independent methods. PCR-TGGE fingerprints analysis of different samples, taken along the olive harvesting season, revealed important variations of the bacterial community structure showing rapid establishment of prevalent bacterial populations. Several bacteria, isolated from OWW, were cultivated, in media containing increasing amounts of polyphenols, in order to select high phenol-degrading strains for the effluent pollutants reduction. Strains PM3 and PM15, affiliated to Raoultella terrigena and Pantoea agglomerans by 16S rRNA gene sequencing, were selected and used for OWW biological treatment under batch conditions in shake flasks cultures. The OWW content of phenols, BOD5, COD and colour, was reduced by 93, 91, 89 and 62%, respectively, permitting effluent disposal and/or reuse with no additional treatments.