Tracking cell proliferation using a nanotechnology based approach
Metadatos
Mostrar el registro completo del ítemAutor
Altea-Manzano, Patricia; Unciti-Broceta, Juan Diego; Cano Cortes, María Victoria; Ruiz-Blas, María Paz; Valero-Griñan, Mª Teresa; Díaz-Mochón, Juan José; Sánchez-Martín, Rosario MaríaEditorial
IOP Publishing
Fecha
2017Referencia bibliográfica
Altea-Manzano P, Unciti-Broceta JD, Cano-Cortes V, Ruiz-Blas MP, Valero-Grinan Teresa M,Diaz-Mochon JJ and Sanchez-Martin R (2017) Tracking cell proliferation using a nanotechnology-based approach. Nanomedicine. 12(13): 1591-1605.
Patrocinador
This research was supported by Marie Curie Career Integration Grants within the 7th European Community Framework Programme (FP7-PEOPLE-2011-CIG-Project Number 294142 and FP7-PEOPLE-2012-CIG-Project Number 322276). This research was partially supported by the Consejería de Economía, Innovación y Ciencia de la Junta de Andalucía (BIO-1778). JDUB thanks Spanish Ministerio de Economía y Competitividad for a Torres Quevedo fellowship (PTQ-13-06046) and MTVG thanks to the Consejería de Economía, Innovación, Ciencia y Empleo de la Junta de Andalucía for an EU funded FP7 postdoc Talentia Fellowship (FP7-TALENTIA- POSTDOC-267226).Resumen
Aim: To develop an efficient nanotechnology fluorescence based method to track cell proliferation to avoid the limitations of current cell-labelling dyes. Material & methods: Synthesis, PEGylation, bifunctionalization and labelling with a fluorophore (Cy5) of 200 nm polystyrene nanoparticles (NPs) were performed. These NPs were characterised and assessed for in vitro long-term monitoring of cell proliferation. Results: The optimisation and validation of this method to track long term cell proliferation assays have been achieved with high reproducibility, without cell cycle disruption. This method has been successfully applied in several adherent and suspension cells including hard-to-transfect cells and isolated human primary lymphocytes. Conclusion: A novel approach to track efficiently cellular proliferation by flow cytometry using fluorescence labelled nanoparticles has been successfully developed.