Exosome secretion by eosinophils: A possible role in asthma pathogenesis
Metadatos
Mostrar el registro completo del ítemAutor
Mazzeo, Carla; Cañas, José Antonio; Zafra, María Paz; Rojas Marco, Ainara; Fernández-Nieto, Mar; Sanz, Verónica; Mittlebrunn, María; Izquierdo, Manuel; Baixaulli, Francesc; Sastre, Joaquín; Del Pozo, VictoriaEditorial
Elsevier
Materia
Asthma CD63 IFN-γ biomarker endosomes exosomes lysobisphosphatidic acid multivesicular bodies secretion eosinophils
Fecha
2015Referencia bibliográfica
Mazzeo C. et al. Exosome secretion by eosinophils: A possible role in asthma pathogenesis. J Allergy Clin Immunol. 2015 Jun;135(6):1603-13. https://doi.org/10.1016/j.jaci.2014.11.026
Patrocinador
Fondo de Investigación Sanitaria–FIS (PS09/00153 and PI12/00691); Carlos III Institute of Health; Conchita Rabago Foundation (MPZ)Resumen
Background
Eosinophils secrete several granules that are involved in the propagation of inflammatory responses in patients with pathologies such as asthma.
Objective
We hypothesized that some of these granules are exosomes, which, when transferred to the recipient cells, could modulate asthma progression.
Methods
Eosinophils were purified from peripheral blood and cultured with or without IFN-γ or eotaxin. Multivesicular bodies (MVBs) in eosinophils were studied by using fluorescence microscopy, transmission electron microscopy (TEM), and flow cytometry. Exosome secretion was measured and exosome characterization was performed with TEM, Western blotting, and NanoSight analysis.
Results
Generation of MVBs in eosinophils was confirmed by using fluorescence microscopy and flow cytometry and corroborated by means of TEM. Having established that eosinophils contain MVBs, our aim was to demonstrate that eosinophils secrete exosomes. To do this, we purified exosomes from culture medium of eosinophils and characterized them. Using Western blot analysis, we demonstrated that eosinophils secreted exosomes and that the discharge of exosomes to extracellular media increases after IFN-γ stimulation. We measured exosome size and quantified exosome production from healthy and asthmatic subjects using nanotracking analysis. We found that exosome production was augmented in asthmatic patients.
Conclusion
Our findings are the first to demonstrate that eosinophils contain functional MVBs and secrete exosomes and that their secretion is increased in asthmatic patients. Thus exosomes might play an important role in the progression of asthma and eventually be considered a biomarker.