CTS987 - Artículos
https://hdl.handle.net/10481/63755
2024-03-28T11:24:17ZIdentification of Trypanosomatids by detecting Single Nucleotide Fingerprints using DNA analysis by dynamic chemistry with MALDI-ToF
https://hdl.handle.net/10481/88617
Identification of Trypanosomatids by detecting Single Nucleotide Fingerprints using DNA analysis by dynamic chemistry with MALDI-ToF
Luque-González, María Angélica; Sánchez Martín, Rosario María; Ortiz González, Matilde; Soriano Rodríguez, Miguel; García Salcedo, José Antonio; Díaz Mochón, Juan José
Protozoan parasites of the Trypanosomatidae family cause severe diseases like Human African Trypanosomiasis, Chagas disease, and Leishmaniasis. Despite existing molecular assays for detection and monitoring, limitations persist. We introduce a novel molecular assay combining dynamic chemistry DNA analysis with MALDI-ToF mass spectrometry, allowing rapid identification and differentiation of Trypanosomatid species with singleplex PCR. This innovative approach enhances diagnostic capabilities and therapeutic monitoring for these diseases.
Direct Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challenges
https://hdl.handle.net/10481/88616
Direct Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challenges
López-Longarela, B; Morrison, EE; Tranter, JD; Chahman-Vos, L; Léonard, JF; Gautier, JC; Laurent, S; Lartigau, A; Boitier, E; Sautier, L; Carmona-Saez, P; Martorell-Marugan, J; Mellanby, RJ; Pernagallo, S; Ilyine, H; Rissin, DM; Duffy, DC; Dear, JW; Diaz Mochon, Juan José
Circulating microRNAs serve as stable and cross-species biomarkers. MicroRNA-122 (miR-122) is a reliable hepatocyte-specific biomarker for drug-induced liver injury (DILI). We developed a dynamic chemical labeling (DCL) assay enabling direct detection of miR-122 in blood, with high specificity and sensitivity from small sample volumes. This assay accurately identifies DILI in humans and animals, offering improved stability and isomiR detection compared to PCR methods.
Amplification-free profiling of microRNA-122 biomarker in DILI patient serums, using the luminex MAGPIX system
https://hdl.handle.net/10481/88615
Amplification-free profiling of microRNA-122 biomarker in DILI patient serums, using the luminex MAGPIX system
Marín Romero, Antonio; Sánchez Martín, Rosario María; Díaz Mochón, Juan José
Dynamic chemical labelling is a single-base specific method to enable detection and quantification of micro-Ribonucleic Acids in biological fluids without extraction and pre-amplification. In this study, dynamic chemical labelling was combined with the Luminex MAGPIX system to profile levels of microRNA-122 biomarker in serum from patients with Drug-Induced Liver Injury.
PCR-free and chemistry-based technology for miR-21 rapid detection directly from tumour cells
https://hdl.handle.net/10481/88591
PCR-free and chemistry-based technology for miR-21 rapid detection directly from tumour cells
Delgado-Gonzalez, Antonio; Robles Remacho, Agustín; Marín Romero, Antonio; De Miguel Pérez, Diego; Guardia Monteagudo, Juan José; Fara, Mario A.; Sánchez Martín, Rosario María; Díaz Mochón, Juan José
miRNAs play pivotal roles in various biological contexts, particularly in disease. Notably, miRNA-21 is implicated in severe pathological conditions, necessitating accurate quantification for medical diagnostics. Existing miRNA quantification techniques often fall short. Here, we introduce a novel, dynamic chemistry-based, PCR-free method for rapid and cost-effective miRNA-21 identification and quantification directly from tumor cells, employing FACS and fluorescent microplate assays. This approach, utilizing bead-based reagents, enables precise quantification of miR-21 molecules in MDA-MB-468 and H1975 tumor cells.
Hybrid Fluorescent Mass-Tag Nanotrackers as Universal Reagents for Long-Term Live-Cell Barcoding
https://hdl.handle.net/10481/88581
Hybrid Fluorescent Mass-Tag Nanotrackers as Universal Reagents for Long-Term Live-Cell Barcoding
Delgado-Gonzalez, Antonio; Laz Ruiz, José Antonio; Cano Cortes, María Victoria; Díaz Mochón, Juan José; Sánchez Martín, Rosario María
Cell barcoding and pooling are essential to reduce technical variability in multiplex technologies. While fluorescent barcoding is common in flow cytometry, current mass-tag barcoding methods are unsuitable for live cells. This study presents fluorescent palladium-based nanotrackers for live cell barcoding, compatible with flow and mass cytometry, enabling long-term drug studies with consistent labeling.