https://hdl.handle.net/10481/26258 2026-04-03T23:39:03Z https://hdl.handle.net/10481/27469 Hook effect in radioligand assay for Anti Glutamic Acid Decarboxylase (Anti-GAD65). Influence of temperature and physicochemical interpretation Peris-Conejero, T.; Olivares-Pallerols, R.; García-Ruiz, H.; Moreno-Frigols, J.I. Background: Radioligand assay is one of the principal methods used for analytical determination of the Anti-GAD65 concentration. We studied the influence of temperature on the calibration curves obtained by such a method Matherial and Methods: We used a commercially available RIA kit for Anti-GAD65 and a gamma counter. Data was analyzed using Statistica software. Results and Discusion: Activities bound to the antibody increase with temperature. There was a decrease in activity for high concentrations attributable to the "hook effect". We propose a simple physicochemical model that justifies satisfactorily the results.; Objetivo: El análisis por radioligando es uno de los métodos principales utilizados en la determinación analítica del Anti-GAD65. Se ha estudiado la influencia de la temperatura sobre las gráficas de calibración obtenidas por dicha técnica. Material y Métodos: Usamos un kit comercial para Anti-GAD65 y un contador gamma. Los resultados son analizados mediante el programa Statistica. Resultados y Discusión: Las actividades ligadas al anticuerpo aumentan con la temperatura. Se observa una disminución de la actividad para altas concentraciones atribuible al llamado “efecto anzuelo”. Se propone un sencillo modelo fisicoquímico que justifica satisfactoriamente los resultados https://hdl.handle.net/10481/27468 Development and validation of enzymatic method for the determination of alcohol in immunoglobulin and albumin Carrillo-Cabrera, D.; González-Farah, A.; Rodríguez-Bernal, J.; Suárez-Pérez, Y.; Fernández-Cervera, M.; Veliz Rivera, J. Se desarrolló y validó un método enzimático para la cuantificación del contenido de alcohol en los IFAs de Inmunoglobulina y Albúmina. En ambos casos el procedimiento analítico resultó lineal, exacto, preciso y específico para el control de calidad. Se demostró que el método fue lineal en el rango de 6.0 a 19.0 mg de alcohol/g de proteína para la albúmina y de 9.2 a 27.6 mg de alcohol/g de proteína para la inmunoglobulina, respectivamente. El método sugerido se aplicó con éxito en la determinación del contenido de alcohol como impureza en lotes industriales.; An enzymatic method for the quantification of alcohol content in immunoglobulin and albumin was developed and validated. In both materials, the analytical procedure was linear, accurate, precise and specific. The method was linear in the range from 6.0 to 19.0 mg of alcohol/g of protein to albumin and to immunoglobulin from 9.2 to 27.6 mg of alcohol/g of protein, respectively. The proposed method was applied successfully in industrial batches for the determination of the alcohol content as impurity. https://hdl.handle.net/10481/27467 Formulation and evaluation of sustained release microspheres of rosin containing aceclofenac Lakshmana Prabu, S.; Shirwaikar, A.A.; Shirwaikar, A.; Kumari, A. Aceclofenac was microencapsulated using rosin by o/w emulsion solvent evaporation technique. The effect of three formulation variables including the drug:polymer ratio, emulsifier (polyvinyl alcohol) concentration and organic solvent (dichloromethane) volume were examined. The prepared batches were characterized for microspheres particle size distribution, encapsulation efficiency and in vitro release behavior. The study reveals that drug:polymer ratio had a considerable effect on the entrapment efficiency, however particle size distribution of microspheres was more dependent on the volume of dichloromethane and polyvinyl alcohol concentration rather than on the drug: polymer ratio. Drug, polymer concentrations were varied to obtain optimum release profile for sustaining the action of the drug. https://hdl.handle.net/10481/27466 Drug delivery systems based on poly(e-caprolactone) for cancer treatment Sáez-Fernández, E.; Ruiz Martínez, María A.; Arias Mediano, José Luis Chemotherapy agents have little or no specificity over cancer cells, resulting in low therapeutic concentrations at the tumor site (a consequence of a broad systemic distribution), and severe side effects. With the aim of avoiding cancer therapy failure, several approaches such as design of new anticancer drugs, chemical engineering of conventional drugs and development of drug delivery systems have been proposed. The objective is to enhance drug localization at the tumor region (by controlling its biodistribution profile) and, therefore, to increase the anti-tumor efficacy (even in multi-drug resistant tumors), while reducing systemic side effects. One of the most promising approaches to the problem is the development of drug nanocarriers based on the polymer poly(e-caprolactone). In this review we will focus our attention on these polymeric colloids, particularly on the most significant characteristics and formulation procedures, and on their use as nanoplatforms for the delivery of chemotherapy agents to the tumor site. Furthermore, the most recent in vitro and in vivo investigations on the subject are extensively reviewed. https://hdl.handle.net/10481/27464 Adjudication of new community pharmacies: comparison of ranking criteria Ríos, L.M.; Garrigues, T.M.; Martín, A.; Muelas, J.A. This article studies the differences between the models of candidate classification for the adjudication of new pharmacies in the different Spanish self-governing regions. The objective of this study is to analyze the profile of the selected professional. Generally, the ranking takes into account the professional activity as pharmacist, the pre-graduate formation as well as the postgraduate, with different weights. Some of the 15 autonomies include as merits the cooficial languages, an optional written test and special situations as unemployment. In general, the criteria try to find out the best profile of pharmacist based on the professional experience and the postgraduate formation.