https://hdl.handle.net/10481/68140 2026-04-18T08:18:58Z 2026-04-18T08:18:58Z Zarza Arribas, María Alonso Santor, Juan Ernesto https://hdl.handle.net/10481/69322 2021-06-21T11:23:01Z

Sarcoidosis and primary hyperparathyroidism Zarza Arribas, María; Alonso Santor, Juan Ernesto The etiopathogenical relationship between sarcoidosis and primary hyperparathyroidism is not yet fully defined. This coexistence remains rare in medical literature despite their relatively high prevalence. Therefore, this potential association should be considered in the differential diagnosis of a patient with sarcoidosis presenting symptoms of hyperparathyroidism. In this paper we describe the case of a patient diagnosed with sarcoidosis who presented an initial episode of symptomatic hypercalcemia successfully treated with corticoids. After remaining asymptomatic for years, hypercalcemia reappears in several tests. The complete study on calcium metabolism leads the diagnosis toward primary hyperparathyroidism. This case is relevant as it emphasizes the need to avoid attributing to hypercalcemia a reaction proper to sarcoidosis. Thus, it is intended to highlight the need to approach differential diagnosis of hypercalcemia instead of administering a treatment with corticoids.

Castro, L. https://hdl.handle.net/10481/69321 2021-06-21T11:18:27Z

Prevention and treatment of burns in diabetic patients' feet Castro, L. THE LOSS OF SENSIBILITY CAUSED BY DIABETIC NEUROPATHY REQUIRES THE PATIENT'S SELF-CARE TO AVOID FAINT WOUNDS. IN THIS CASE, THE PATIENT SUFFERS BURNS DESPITE BEING EDUCATED IN THE MATTER. THIS CAN BE ATTRIBUTED TO THE THERMAL SOURCE'S RARITY (SAND). THE PATIENT DOES NOT IDENTIFY THE RISK BECAUSE HE DOES NOT RELATE IT TO HIS PATHOLOGY. THIS CLINICAL CASE IS AIMED AT STRESSING THE NEED FOR A PROPER EDUCATION IN CASES OF DIABETIC NEUROPATHY, INCLUDING THE RISKS THAT, ALTHOUGH INFREQUENT, ARE REMARKABLE.

Moya Ramírez, Miguel Ángel Sánchez Martín, Victoria Herrera Merchán, Antonio Medina Vico, Pedro Pablo Cuadros Celorrio, Marta Eugenia https://hdl.handle.net/10481/69319 2021-06-21T11:13:20Z

Choosing the right cell line for rectal cancer research Moya Ramírez, Miguel Ángel; Sánchez Martín, Victoria; Herrera Merchán, Antonio; Medina Vico, Pedro Pablo; Cuadros Celorrio, Marta Eugenia Up to date no effective method exists that predicts response to preoperative chemoradiation (CRT) in locally advanced rectal cancer (LARC). Nevertheless, identification of patients who have a higher likelihood of responding to preoperative CRT could be crucial in decreasing treatment morbidity and avoiding expensive and time-consuming treatments. Using the Gng4, c-Myc, Pola1, and Rrm1 signature, we were able to establish a model to predict response to CRT in rectal cancer with a sensitivity of 60% and 100% specificity. The aim of this study was to characterize c-Myc status in DNA, RNA and protein levels in 3 tumoral cell lines (SW480, SW620 and SW837) to establish the best cell line model and, subsequently, carry out genome silencing of c-Myc by means of RNA interference (iRNA). To study the expression levels of c-Myc, we used Polymerase Chain Reaction (PCR) amplifications and sequencing; quantitative real time PCR (qRT-PCR); and western blot analysis in each cell line. SW480 and SW620 showed a variation A > G in exon 2, which caused a substitution of aspargine to serine, and SW837 revealed a G > A transition in the same, which caused a mutation at codon 92. The three cell lines expressed c-Myc mRNA. SW837 showed a decrease of c-Myc expression levels compared with SW480, and SW620. At protein level, SW620 showed the highest expression of c-Myc. According to the results obtained, we can perform c-Myc gene silencing experiments to analyze the role of this biomarker in response to treatment.

González Díez, Manuel Guerra Librero Rite, Ana González Gil, Beatriz Escames Rosa, Germaine https://hdl.handle.net/10481/69318 2022-02-16T12:12:19Z

Oncostatic effect of melatonin in head and neck cancer cells: clonogenic assay González Díez, Manuel; Guerra Librero Rite, Ana; González Gil, Beatriz; Escames Rosa, Germaine Background: The oncostatic effect of melatonin has been previously described among different neoplastic types. One of these is head and neck squamous cell cancer (HNSCC) with a high rate of mortality and morbidity. Melatonin (aMT) could cause cell death in this neoplastic cell type. To determine this, we performed a clonogenic assay with CAL-27 cells treated with melatonin and/or radiation. Methods: Cells were plated in a 6-well plate, with 800 cells per well. Assays were performed at least twice and each time in triplicates. Cells were allowed to grow 15 days to form colonies of at least 50 cells each one. Cells were treated with melatonin (100, 500, 1000, 1500 and 2000 µM) alone or in combination with irradiation (8 Gy). To visualize colonies, cells were fixed in 100 % ethanol on days 12, 13, 14 and 15 after they were plated and stained with crystal violet solution. Colonies were scored with Image J Software. Results: The results clearly show that melatonin inhibits colony growth of CAL-27 cells in a dosedependent manner in the groups treated with melatonin alone 1500 µM or in combination with irradiation. Conclusion: The results show the capability of aMT to prevent colony growth and causing cell death on CAL-27 cancer cells, especially when combined with radiation. This is consistent with previous studies on aMT oncostatic effects and suggests that usage of aMT in vivo should have future clinical application.