CTS242 - Artículoshttps://hdl.handle.net/10481/389362024-03-29T07:19:22Z2024-03-29T07:19:22ZDexamethasone and zinc loaded polymeric nanoparticles reinforce and remineralize coronal dentin. A morpho-histological and dynamic-biomechanical study.Toledano, ManuelOsorio Ruiz, María EstrellaSánchez Aguilera, FátimaOsorio Ruiz, María EstrellaToledano, RaquelLópez-López, Modesto T.Lunch, Christopher D.Osorio Ruiz, Raquelhttps://hdl.handle.net/10481/810122023-04-11T11:57:23ZDexamethasone and zinc loaded polymeric nanoparticles reinforce and remineralize coronal dentin. A morpho-histological and dynamic-biomechanical study.
Toledano, Manuel; Osorio Ruiz, María Estrella; Sánchez Aguilera, Fátima; Osorio Ruiz, María Estrella; Toledano, Raquel; López-López, Modesto T.; Lunch, Christopher D.; Osorio Ruiz, Raquel
Objective: To investigate the effect of novel polymeric nanoparticles (NPs) doped with dexamethasone (Dex) on viscoelasticity, crystallinity and ultra-nanostructure of the formed hydroxyapatite after NPs dentin infiltration.
Methods: Undoped-NPs, Dex-doped NPs (Dex-NPs) and zinc-doped-Dex-NPs (Zn-Dex-NPs) were tested at dentin, after 24 h and 21 d. A control group without NPs was included. Coronal dentin surfaces were studied by nano-dynamic mechanical analysis measurements, atomic force microscopy, X-ray diffraction and transmission electron microscopy. Mean and standard deviation were analyzed by ANOVA and Student-Newman-Keuls multiple comparisons (p < 0.05).
Results: At 21 d of storage time, both groups doped with Dex exhibited the highest complex, storage and loss moduli among groups. Zn-Dex-NPs and Dex-NPs promoted the highest and lowest tan delta values, respectively. Dex-NPs contributed to increase the fibril diameters of dentin collagen over time. Dentin surfaces treated with Zn-Dex-NPs attained the lowest nano-roughness values, provoked the highest crystallinity, and produced the longest and shortest crystallite and grain size. These new crystals organized with randomly oriented lattices. Dex-NPs induced the highest microstrain. Crystalline and amorphous matter was present in the mineral precipitates of all groups, but Zn and Dex loaded NPs helped to increase crystallinity.
Significance: Dentin treated with Zn-Dex-NPs improved crystallographic and atomic order, providing structural stability, high mechanical performance and tissue maturation. Amorphous content was also present, so high hydroxyapatite solubility, bioactivity and remineralizing activity due to the high ion-rich environment took place in the infiltrated dentin.
Polymeric zinc-doped nanoparticles for high performance in restorative dentistryToledano Pérez, ManuelVallecillo-Rivas, MartaSánchez Aguilera, FátimaOsorio Ruiz, María EstrellaOsorio Ruiz, Raquelhttps://hdl.handle.net/10481/672572021-06-15T13:08:57ZPolymeric zinc-doped nanoparticles for high performance in restorative dentistry
Toledano Pérez, Manuel; Vallecillo-Rivas, Marta; Sánchez Aguilera, Fátima; Osorio Ruiz, María Estrella; Osorio Ruiz, Raquel
Objectives: The aim was to state the different applications and the effectiveness of polymeric zinc-doped nanoparticles to achieve dentin remineralization.
Data, Sources and Study selection: Literature search was conducted using electronic databases, such as PubMed, MEDLINE, DIMDI, Embase, Scopus and Web of Science. A narrative exploratory review was undertaken.
Conclusions: Polymeric nanospheres (NPs) were efficiently loaded with zinc. NPs sequestered calcium and phosphate in the presence of silicon, and remained effectively embedded at the hybrid layer. NPs incorporation did not alter bond strength and inhibited MMP-mediated dentin collagen degradation. Zn-loaded NPs remineralized the hybrid layer inducing a generalized low-carbonate substitute apatite precipitation, chemically crystalline with some amorphous components, and an increase in mechanical properties was also promoted. Viscoelastic analysis determined that dentin infiltrated with Zn-NPs released the stress by breaking the resin-dentin interface and creating specific mineral formations in response to the energy dissipation. Bacteria were scarcely encountered at the resin-dentin interface. The combined antibacterial and remineralizing effects, when Zn-NPs were applied, reduced biofilm formation. Zn-NPs application at both cervical and radicular dentin attained the lowest microleakage and also promoted durable sealing ability. The new zinc-based salt minerals generated covered the dentin surface totally occluding cracks, porosities and dentinal tubules.
Clinical significance: Zinc-doped NPs are proposed for effective dentin remineralization and tubular occlusion. This offers new strategies for regeneration of eroded cervical dentin, effective treatment of dentin hypersensitivity and in endodontically treated teeth previous to the canal filling. Zn-NPs also do reduce biofilm formation due to antibacterial properties.
Polymeric nanoparticles protect the resin-dentin bonded interface from cariogenic biofilm degradationToledano Osorio, ManuelOsorio Ruiz, RaquelSánchez Aguilera, FátimaMedina Castillo, Antonio LuisToledano Pérez, ManuelOsorio Ruiz, María EstrellaAcosta, SergioChen, RuoqiongAparicio, Conradohttps://hdl.handle.net/10481/672552021-12-23T13:00:26ZPolymeric nanoparticles protect the resin-dentin bonded interface from cariogenic biofilm degradation
Toledano Osorio, Manuel; Osorio Ruiz, Raquel; Sánchez Aguilera, Fátima; Medina Castillo, Antonio Luis; Toledano Pérez, Manuel; Osorio Ruiz, María Estrella; Acosta, Sergio; Chen, Ruoqiong; Aparicio, Conrado
The objective was to assess doxycycline (Dox) and zinc (Zn) doped nanoparticles' (NPs) potential to protect the resin-dentin interface from cariogenic biofilm. Three groups ofpolymeric NPs were tested: unloaded, loaded with zinc and with doxycycline. NPs were appliedafter dentin etching. The disks were exposed to a cariogenic biofilm challenge in a Drip-FlowReactor during 72 h and 7 d. Half of the specimens were not subjected to biofilm formation butstored 72 h and 7 d. LIVE/DEAD® viability assay, nano-dynamic mechanicalassessment, Raman spectroscopy and field emission electron microscopy (FESEM) analysiswere performed. The measured bacterial death rates, at 7 d were 46% for the control group, 51%for the undoped-NPs, 32% for Dox-NPs, and 87% for Zn-NPs; being total detected bacteriareduced five times in the Dox-NPs group. Zn-NPs treated samples reached, in general, thehighest complex modulus values at the resin-dentin interface over time. Regarding the mineralcontent, Zn-NPs-treated dentin interfaces showed the highest mineralization degree associatedto the phosphate peak and the relative mineral concentration. FESEM images after Zn-NPsapplication permitted to observe remineralization of the etched and non-resin infiltratedcollagen layer, and bacteria were scarcely encountered. The combined antibacterial andremineralizing effects, when Zn-NPs were applied, reduced biofilm formation. Dox-NPs exertedan antibacterial role but did not remineralize the bonded interface. Undoped-NPs did notimprove the properties of the interfaces. Application of Zn-doped NPs during the bondingprocedure is encouraged.
Testing Active Membranes for Bone Regeneration: a ReviewToledano Osorio, ManuelManzano-Moreno, Francisco JavierRuiz Rodríguez, ConcepciónToledano Pérez, ManuelOsorio Ruiz, Raquelhttps://hdl.handle.net/10481/671082023-05-16T08:24:06ZTesting Active Membranes for Bone Regeneration: a Review
Toledano Osorio, Manuel; Manzano-Moreno, Francisco Javier; Ruiz Rodríguez, Concepción; Toledano Pérez, Manuel; Osorio Ruiz, Raquel
Objectives:Maxillofacial bone defectsare the main hindering conditions for traditional dental implant strategies. Guided Bone Regeneration (GBR) is used to handle this situation. The principle of GBR is to use a membrane to prevent the colonization of soft tissue cells of the bone defect and favors the migration of osteogenic linages. Currentmembranes do not completely fulfill the requirements that an optimal membrane should have, sometimes resulting in non-predictable results. Thus, the need to develop an ideal membrane to perform this duty is clear. Recent developments in bio-manufacturing are driving innovations in membranes technology permitting the active participation of the membrane in the healing and regenerative process trough native tissue mimicking, drug-delivery and cells interaction, away from being a passive barrier. New membranes features need specific evaluation techniques, beyond the International Standard for membrane materials (last reviewed in 2004), being this the rationale for the present review.Nanotechnology application has completely shifted the way of analyzing structural characterization. New progresses on osteoimmmunomodulation have also switched the understanding of cells-membranes interaction. Data and Sources:To propose an updated protocol for GBR membranes evaluation, critical reading of therelevant published literature was carried out after a MEDLINE/PubMed database search. Conclusions:The main findings are that a potential active membrane should be assessed in its nanostructure, physicochemical and nanomechanical properties,bioactivityand antibacterial,osteoblasts proliferation, differentiation and mineralization. Immunomodulationtesting for macrophages recruitmentand M2 phenotype promotion in osteoblasts co-culture has to beachieved to completely analyze membranes/tissue interactions.
This work was supported by: 1)the Ministry of Economy and Competitiveness and European Regional Development Fund [Project MAT2017-85999-P MINECO/AEI/FEDER/UE],2) University of Granada/Regional Government of Andalusia Research Fund from Spain and European Regional Development Fund (A-BIO-157-UGR-18/FEDER).This research is part of M.T-O.’s PhD research study.
Zn-doping of silicate and hydroxyapatite-based cements: dentin mechanobiology and bioactivityToledano Pérez, ManuelOsorio Ruiz, RaquelVallecillo-Rivas, MartaOsorio Ruiz, María EstrellaLynch, Christopher D.Aguilera, Fátima S.Toledano, RaquelSauro, Salvatorehttps://hdl.handle.net/10481/671052023-05-16T08:24:14ZZn-doping of silicate and hydroxyapatite-based cements: dentin mechanobiology and bioactivity
Toledano Pérez, Manuel; Osorio Ruiz, Raquel; Vallecillo-Rivas, Marta; Osorio Ruiz, María Estrella; Lynch, Christopher D.; Aguilera, Fátima S.; Toledano, Raquel; Sauro, Salvatore
The objective was to state zinc contribution in the effectiveness of novel zinc-doped dentin cements to achieve dentin remineralization, throughout a literature or narrative exploratory review. Literature search was conducted using electronic databases, such as PubMed, MEDLINE, DIMDI, Embase, Scopus and Web of Science. Both zinc-doping silicate and hydroxyapatite-based cements provoked an increase of both bioactivity and intrafibrillar mineralization of dentin. Zinc-doped hydroxyapatite-based cements (oxipatite) also induced an increase in values of dentin nano-hardness, Young’s modulus and dentin resistance to deformation. From Raman analyses, it was stated higher intensity of phosphate peaks and crystallinity as markers of dentin calcification, in the presence of zinc. Zinc-based salt formations produced low microleakage and permeability values with hermetically sealed tubules at radicular dentin. Dentin treated with oxipatite attained preferred crystal grain orientation with polycrystalline lattices. Thereby, oxipatite mechanically reinforced dentin structure, by remineralization. Dentin treated with oxipatite produced immature crystallites formations, accounting for high hydroxyapatite solubility, instability and enhanced remineralizing activity.
This work was supported by the Ministry of Economy and Competitiveness and European Regional Development Fund [MAT2017-85999PMINECO/AEI/FEDER/UE]. The authors affirm that no actual or potential conflict of interest including any financial, personal or other relationships with other people or organizations within three years of beginning the submitted work that could inappropriately influence, or be perceived to influence, their work. Any other potential conflict of interest is disclosed.