DH - Artículoshttps://hdl.handle.net/10481/299242024-03-28T11:46:09Z2024-03-28T11:46:09ZScleral surgical repair through the use of nanostructured fibrin/agarose-based films in rabbits.Carriel Araya, VíctorVizcaíno López, GersonChato Astrain, JesúsDurand Herrera, DanielAlaminos Mingorance, MiguelCampos Muñoz, Antonio JesúsSánchez-Montesinos García, IndalecioCampos Sánchez, Fernandohttps://hdl.handle.net/10481/901732024-03-22T07:54:56ZScleral surgical repair through the use of nanostructured fibrin/agarose-based films in rabbits.
Carriel Araya, Víctor; Vizcaíno López, Gerson; Chato Astrain, Jesús; Durand Herrera, Daniel; Alaminos Mingorance, Miguel; Campos Muñoz, Antonio Jesús; Sánchez-Montesinos García, Indalecio; Campos Sánchez, Fernando
Scleral defects can result as a consequence of trauma, infectious diseases or cancer and surgical repair with
allogeneic scleral grafts can be required. However, this method has limitations and novel alternatives are
needed. Here, the efficacy of acellular nanostructured fibrin-agarose hydrogel-based substitutes (NFAH) in the
repair of scleral defects in rabbits was studied. For this, scleral defects of 5-mm diameter were made on 18
adult-male New Zealand rabbits and repaired with acellular NFAH, NFAH crosslinked with genipin (NFAH-GP) or
glutaraldehyde (NFAH-GA), allogeneic scleral grafts as control (C-CTR) or not repaired (negative control N-CTR)
(n=3 each). Macroscopic and histological analyses were performed after 40-days. Macroscopy confirmed the
repair of all defects in a comparable manner than the C-CTR. Histology showed no degradation nor integration in
C-CTR while NFAH-GP and NFAH-GA biomaterials were encapsulated by connective and inflammatory tissues
with partial biodegradation. The NFAH were fully biodegraded and replaced by a loose connective tissue and
sclera covering the defects. This in vivo study demonstrated that the NFAH are a promising biocompatible and
pro-regenerative alternative to the use of allogeneic cadaveric grafts. However, large defects and long-term
studies are needed to demonstrate the potential clinical usefulness of these substitutes.
This study was supported by Consejería de Salud y Familias, Regional Ministry of Health, Junta de Andalucía, Spain, Grant CS PI-0400-2016 and by Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica, Ministerio de Economía y Competitividad, Spain, Grant FIS PI17/391, co-financed by “Fondo Europeo de Desarrollo Regional (FEDER),” European Union.
Detergent-based decellularized peripheral nerve allografts: An in vivo preclinical study in the rat sciatic nerve injury modelChato Astrain, JesúsPhilips, CharlotCampos Sánchez, FernandoDurand Herrera, DanielGarcía García, Óscar DaríoRoosens, AnneliesAlaminos Mingorance, MiguelCampos Muñoz, Antonio JesúsCarriel Araya, Víctorhttps://hdl.handle.net/10481/901172024-03-20T13:11:01ZDetergent-based decellularized peripheral nerve allografts: An in vivo preclinical study in the rat sciatic nerve injury model
Chato Astrain, Jesús; Philips, Charlot; Campos Sánchez, Fernando; Durand Herrera, Daniel; García García, Óscar Darío; Roosens, Annelies; Alaminos Mingorance, Miguel; Campos Muñoz, Antonio Jesús; Carriel Araya, Víctor
Nerve autograft is the gold standard technique to repair critical nerve defects, but efficient alternatives are needed. The present study evaluated the suitability of our novel Roosens-based (RSN) decellularized peripheral nerve allografts (DPNAs) in the repair of 10-mm sciatic nerve defect in rats at the functional and histological levels after 12 weeks. These DPNAs were compared with the autograft technique (AUTO) and Sondell (SD) or Hudson (HD) based DPNAs. Clinical and functional assessments demonstrated a partial regeneration in all operated animals. RSN-based DPNAs results were comparable with SD and HD groups and closely comparable with the AUTO group without significant differences (p > .05). Overall hematological studies confirmed the biocompatibility of grafted DPNAs. In addition, biochemistry revealed some signs of muscle affection in all operated animals. These results were confirmed by the loss of weight and volume of the muscle and by muscle histology, especially in DPNAs. Histology of repaired nerves confirmed an active nerve tissue regeneration and partial myelination along with the implanted grafts, being the results obtained with HD and RSN-based DPNAs comparable with the AUTO group. Finally, this in vivo study suggests that our novel RSN-based DPNAs supported a comparable tissue
regeneration, along the 10-mm nerve gap, after 12-week follow-up to HD DPNAs, and both were superior to SD group and closely comparable with autograft technique. However, further improvements are needed to overcome the efficacy of the nerve autograft technique.
Este estudio ha sido financiado por el "Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica, Ministerio de Economía y Competitividad (Instituto de Salud Carlos III), y cofinanciado por el "Fondo Europeo de Desarrollo Regional (FEDER) Unión Europea", Subvención nº FIS PI17-0393, y por el "Programa Operativo FEDER Andalucía 2014-2020, I +D +I, Frontera" Subvención nº A-CTS-488-UGR18. Todo el estudio se realizó en las instalaciones del Grupo de Ingeniería Tisular (CTS-115) de la Universidad de Granada y en la Unidad Experimental del Hospital Universitario Virgen de las Nieves de Granada, España. Este estudio forma parte de la Tesis Doctoral de Jesús Chato-Astrain.
The challenge of discovering the threshold concepts of medical research areas: a bibliometrics-based approachSantisteban-Espejo, AntonioMoral-Muñoz, Jose AntonioMartín Piedra, Miguel ÁngelCampos Muñoz, Antonio Jesúshttps://hdl.handle.net/10481/900732024-03-18T10:58:39ZThe challenge of discovering the threshold concepts of medical research areas: a bibliometrics-based approach
Santisteban-Espejo, Antonio; Moral-Muñoz, Jose Antonio; Martín Piedra, Miguel Ángel; Campos Muñoz, Antonio Jesús
During the last XX century, several changes were applied to traditional educational methods, positioning the student as a central actor in the learning process. One of the pedagogical theories developed was the Threshold Concepts (TC) educational framework, based on education as a space of uncertainty, where the student needs to learn a certain concept or learning experiences that allow developing a new way of thinking. In medical education, written accounts about significant learning experiences, analysis of practice essays and semi-structured focus groups interviews have been applied to identify TC. In that way, our hypothesis is that the use of bibliometrics, as a tool to discover hidden relations between keywords, can overcome traditional difficulties related to TC identification. Keywords are applied to highlight the content of a digital object; they are concepts with a special meaning, similar to TC. Our challenge is to identify the bibliometric indexes that are able to show the relationship between the keywords that make them TC, especially in a medical context. In previous scientific literature, several methods were applied, mainly based on qualitative assessment. In this sense, we propose a quantitative, objective, and reproducible approach that can enrich the learning process from a scientific-based perspective.
Expanded Differentiation Capability of Human Wharton's Jelly Stem Cells Toward Pluripotency: A Systematic ReviewGarzón Bello, Ingrid JohannaChato Astrain, JesúsCampos Sánchez, FernandoFernández Valadés, RicardoSánchez-Montesinos García, IndalecioCampos Muñoz, Antonio JesúsAlaminos Mingorance, MiguelD'Souza, Rena N.Martín Piedra, Miguel Ángelhttps://hdl.handle.net/10481/899672024-03-14T07:48:07ZExpanded Differentiation Capability of Human Wharton's Jelly Stem Cells Toward Pluripotency: A Systematic Review
Garzón Bello, Ingrid Johanna; Chato Astrain, Jesús; Campos Sánchez, Fernando; Fernández Valadés, Ricardo; Sánchez-Montesinos García, Indalecio; Campos Muñoz, Antonio Jesús; Alaminos Mingorance, Miguel; D'Souza, Rena N.; Martín Piedra, Miguel Ángel
Human Wharton’s jelly stem cells (HWJSC) can be efficiently isolated from the umbilical cord, and numerous reports have demonstrated that these cells can differentiate into several cell lineages. This fact, coupled with the high proliferation potential of HWJSC, make them a promising source of stem cells for use in tissue engineering and regenerative medicine. However, their real potentiality has not been established to date. In the present study we carried out a systematic review to determine the multilineage differentiation potential of HWJSC. After a systematic literature search we selected 32 publications focused on the differentiation potential of these cells. Analysis of these studies showed that HWJSC display expanded differentiation potential towards some cell types corresponding to all three embryonic cell layers (ectodermal, mesodermal and endodermal), which is consistent with their constitutive expression of key pluripotency markers such as OCT4, SOX2 and NANOG, and the embryonic marker SSEA4. We conclude that HWJSC can be considered cells in an intermediate state between multipotentiality and pluripotentiality, since their proliferation capability is not unlimited and differentiation to all cell types has not been demonstrated thus far. These findings support the clinical use of HWJSC for the treatment of diseases affecting not only mesoderm-type tissues, but also other cell lineages.
This study was supported by the Spanish Plan Nacional de
Investigación Científica, Desarrollo e Innovación Tecnológica
(I+D+I) of the Spanish Ministry of Economy and Competitiveness (Instituto de Salud Carlos III), grants FIS PI17/0391,
PI18/331 and PI18/332 (co-financed by ERDF-FEDER, European Union).
Nanostructured fibrin-based hydrogel membranes for use as an augmentation strategy in achilles tendon surgical repair in ratsGonzález Quevedo, DavidSánchez Porras, DavidGarcía García, Óscar DaríoChato Astrain, JesúsDiaz-Ramos, MCampos Muñoz, Antonio JesúsCarriel Araya, VíctorCampos Sánchez, Fernandohttps://hdl.handle.net/10481/899152024-03-11T11:21:38ZNanostructured fibrin-based hydrogel membranes for use as an augmentation strategy in achilles tendon surgical repair in rats
González Quevedo, David; Sánchez Porras, David; García García, Óscar Darío; Chato Astrain, Jesús; Diaz-Ramos, M; Campos Muñoz, Antonio Jesús; Carriel Araya, Víctor; Campos Sánchez, Fernando
Hydrogels are polymeric biomaterials characterised by their promising biological and biomechanical
properties, which make them potential alternatives for use in tendon repair. The aim of the present study
was to generate in vitro, and determine the therapeutic efficacy in vivo, of novel nanostructured fibrin-based
hydrogels to be used as an augmentation strategy for the surgical repair of rat Achilles tendon injuries. Fibrin,
fibrin-agarose and fibrin-collagen nanostructured hydrogels (NFH, NFAH and NFCH, respectively) were
generated and their biomechanical properties and cell-biomaterial interactions characterised ex vivo. Achilles
tendon ruptures were created in 24 adult Wistar rats, which were next treated with direct repair (control
group) or direct repair augmented with the generated biomaterials (6 rats/group). After 4 and 8 weeks, the
animals were euthanised for macroscopical and histological analyses. Biomechanical characterisation showed
optimal properties of the biomaterials for use in tendon repair. Moreover, biological analyses confirmed
that tendon-derived fibroblasts were able to adhere to the surface of the generated biomaterials, with high
levels of viability and functionality. In vivo studies demonstrated successful tendon repair in all groups.
Lastly, histological analyses disclosed better tissue and extracellular matrix organisation and alignment with
biomaterial-based augmentation strategies than direct repair, especially when NFAH and NFCH were used.
The present study demonstrated that nanostructured fibrin-collagen hydrogels can be used to enhance the
healing process in the surgical repair of tendon ruptures.