@misc{10481/98776,
year = {2022},
month = {7},
url = {https://hdl.handle.net/10481/98776},
abstract = {Near-infrared (NIR) chemical fluorophores are promising tools for in-vivo imaging in real time but often succumb
to rapid photodegradation. Indocyanine green (ICG) is the only NIR dye with regulatory approval for ocular
imaging in humans; however, ICG, when employed for applications such as labelling immune cells, has limited
sensitivity and does not allow precise detection of specific inflammatory events, for example leukocyte
recruitment during uveitic flare-ups. We investigated the potential use of photostable novel triazole NIR cyanine
(TNC) dyes for detecting and characterising activated T-cell activity within the eye. Three TNC dyes were
evaluated for ocular cytotoxicity in-vitro using a MTT assay and optimised concentrations for intraocular
detection within ex-vivo porcine eyes after topical application or intracameral injections of the dyes. TNC labelled
T-cell tracking experiments and mechanistic studies were also performed in-vitro. TNC-1 and TNC-2 dyes
exhibited greater fluorescence intensity than ICG at 10 μM, whereas TNC-3 was only detectable at 100 μM within
the porcine eye. TNC dyes did not demonstrate any ocular cell toxicity at working concentrations of 10 μM.
CD4+T-cells labelled with TNC-1 or TNC-2 were detected within the porcine eye, with TNC-1 being brighter than
TNC-2. Detection of TNC-1 and TNC-2 into CD4+T-cells was prevented by prior incubation with dynole 34–2 (50
μM), suggesting active uptake of these dyes via dynamin-dependent processes. The present study provides evidence
that TNC dyes are suitable to detect activated CD4+T-cells within the eye with potential as a diagnostic
marker for ocular inflammatory diseases.},
organization = {Research Development Fund from the College of Medical and Dental
Sciences at University of Birmingham},
organization = {Saavedra Fajardo Grant (21124/
SF/19)},
organization = {ERC Consolidator
Grant (DYNAFLUORS, 771443)},
title = {Triazole-derivatized near-infrared cyanine dyes enable local functional fluorescent imaging of ocular inflammation},
doi = {https://doi.org/10.1016/j.bios.2022.114623},
author = {Thomas, Chloe N and Alfahad, Nada and Capewell, Nicholas and Cowley, Jamie and Hickman, Eleanor and Fernández Vargas, Antonio Jesús and Harrison, Neale and Qureshi, Omar S and Bennett, Naomi and Narnes, Nicholas M and Dick, Andrew D and Chu, Colin J and Liu, Xiaoxuan and Denniston, Alastair K and Vendrell, Marc and Hill, Lisa J},
}