@misc{10481/98557,
year = {2024},
month = {12},
url = {https://hdl.handle.net/10481/98557},
abstract = {MicroRNAs (miRNAs) have been recognised as potential biomarkers due to their specific expression patterns in different
biological tissues and their changes in expression under pathological conditions. MicroRNA-122 (miR-122)
is a vertebrate-specific miRNA that is predominantly expressed in the liver and plays an important role in liver metabolism
and development. Dysregulation of miR-122 expression is associated with several liver-related diseases, including
hepatocellular carcinoma and drug-induced liver injury (DILI). Given the potential of miR-122 as a biomarker, its
effective detection is important for accurate diagnosis. However, miRNA detection methods still face challenges,
particularly in terms of accurately identifying miRNA isoforms that may differ by only a single base. Here, with the aim
of advancing accessible methods for the detection of miRNAs with single-base specificity, we have developed
a robust dual nanosystem that leverages the simplicity of click chemistry reactions. Using the dual nanosystem, we
successfully detected miR-122 at single-base resolution using flow cytometry and analysed its expression in various
tumour cell lines with high specificity and strong correlation with TaqMan assay results. We also detected miR-122
in serum and identified four single nucleotide variations in its sequence. The chemistry employed in this dual nanosystem
is highly versatile and offers a promising opportunity to develop nanoparticle-based strategies that incorporate
click chemistry and bioorthogonal chemistry for the detection of miRNAs and their isoforms.},
organization = {NANOCARE 2.0 network
(Grant RED2022-134560-T), funded by MCIN/AEI/10.13039/501100011033},
organization = {Spanish Ministry of Science, Innovation and Universities for PhD
funding (scholarship FPU22/03455)},
organization = {Government of Andalusia
for her postdoctoral fellowship (Postdoc_21_00118)},
organization = {DESTINA Genomics Ltd. Schemes in the Graphical
Abstract and Figure 1 were partially created using BioRender.com},
organization = {MCIN/AEI/10.13039/501100011033
and for the European Union Next Generation EU/PRTR (Grant Number:
PID2019.110987RB.I00, PDC2022.133913.I00 and PID2022-141065OB-I00)},
organization = {European Union’s Horizon 2020 research and innovation program under
the Marie Skłodowska-Curie actions (MSCA-RISE-101007934, diaRNAgnosis)},
publisher = {Springer Nature},
keywords = {MicroRNA},
keywords = {Microribonucleic acid},
keywords = {miR-122},
title = {Click chemistry‑based dual nanosystem for microRNA‑122 detection with single‑base specificity from tumour cells},
doi = {10.1186/s12951-024-03071-6},
author = {Robles Remacho, Agustín and Martos Jamai, Ismael and Tabraue-Chávez, Mavys and Aguilar González, Araceli and Laz Ruiz, José Antonio and Cano Cortes, María Victoria and López Delgado, Francisco Javier and Guardia Monteagudo, Juan José and Pernagallo, Salvatore and Diaz Mochon, Juan José and Sánchez Martín, Rosario María},
}