@misc{10481/98512,
year = {2024},
month = {12},
url = {https://hdl.handle.net/10481/98512},
abstract = {In many human rights and criminal contexts, skeletal remains are often the only
available samples, and they present a significant challenge for forensic DNA profiling
due to DNA degradation. Ancient DNA methods, particularly capture hybridization
enrichment, have been proposed for dealing with severely degraded bones, given their
capacity to yield results in ancient remains. Background/Objectives: This paper aims
to test the efficacy of genome-wide capture enrichment on degraded forensic human
remains compared to autosomal STRs analysis. Methods: Six highly degraded human
bones from the Spanish Civil War (1936–1939) were quantified with Quantifiler™ Trio and
amplified with GlobalFiler™. Independently, partially UDG-treated double-stranded DNA
libraries were generated and shotgun sequenced to screen for endogenous human DNA
content. Subsequently, libraries were enriched with the Twist Bioscience “Twist Ancient
DNA” reagent enrichment kit, which had not been previously tested for forensic purposes.
Results: The results show that the samples behave similarly with both approaches (wellpreserved
samples yield good results). However, capture enrichment provides some new
relevant insights, suggesting that its implementation in current NGS forensic platforms
could be beneficial. Conclusions: Shotgun results show that the analyzed samples exhibit
the same characteristics as ancient DNA samples in terms of DNA fragmentation and
molecular damage, which may enhance the value of this approach when authenticating the
endogenous DNA of forensic samples.},
organization = {Spanish Ministry of Universities for funding the development
of his PhD [FPU 20/01967, EST23/0110]},
organization = {Foundation for Science
and Technology, I.P./MCTES (PTDC/HAR-ARQ/6273/2020) for funding the development of his
postdoctoral fellowship through the Portuguese National Funds (PIDDAC)},
organization = {Open access publication
funded by the University of Granada (LS2024.1)},
publisher = {MDPI},
keywords = {ancient DNA},
keywords = {capture enrichment},
keywords = {human remains},
title = {Exploring the Potential of Genome-Wide Hybridization Capture Enrichment for Forensic DNA Profiling of Degraded Bones},
doi = {10.3390/genes16010023},
author = {Haarkötter Cardoso, Christian and Roca Rada, Xavier and Sáiz Guinaldo, María and Vinueza Espinosa, Diana C. and Gálvez Escolano, Xiomara and Medina Lozano, María Isabel and Díaz Ruiz, Daniel and Álvarez Merino, Juan Carlos and Llamas, Bastien and Lorente Acosta, José Antonio and Austin, Jeremy},
}