@misc{10481/98345,
year = {2024},
month = {12},
url = {https://hdl.handle.net/10481/98345},
abstract = {Background/Objectives: Interstitial fibrosis/tubular atrophy in kidney transplantation is
an unspecific lesion induced by immune and non-immune factors, which determines the progression
of chronic kidney disease. Hydroxyproline is an imino acid that is part of the molecule of collagen.
The aim of this study was to assess hydroxyproline in urine microvesicles as a marker of fibrosis
in the renal transplant patient. Patients and Methods: An observational cross-sectional study was
conducted on 46 renal transplant patients who had undergone renal biopsy with diagnostic intention,
as well as 19 healthy controls. Clinical, histological, and laboratory variables were collected at the
time of marker determination and renal function was analyzed 2 years later. Hydroxyproline was
measured in urine microvesicles. Results: Renal transplant patients showed a higher microvesicular
concentration of hydroxyproline compared to the control group, with the following medians
(interquartile range (IQR)): 28.024 (5.53) ng/mL vs. 2.51 (1.16) ng/mL, p < 0.001. In the transplanted
patients, patients in whom biopsy showed some score of total cortical parenchymal inflammation
(ti) displayed a significantly higher concentration of hydroxyproline in urine microvesicles than
those patients who did not score for cortical parenchymal inflammation (29.91 ± 2.797 ng/mL vs.
22.72 ± 8.697 ng/mL, p = 0.034). No significant correlation was observed between urinary markers
and serum creatinine, calcium, and parathyroid hormone (PTH). Conclusions: The concentration
of hydroxyproline in urinary microvesicles increased in renal transplant patients relative to healthy
controls. Hydroxyproline in urinary microvesicles is a marker of chronic renal inflammation in
transplanted patients, and further studies are required to confirm this finding in other pathologies, as
well as the association with fibrosis and the evolution of renal function.},
organization = {Carlos III Health Institute of Spain, grant number
PI18/01715},
organization = {Cooperative Research Network, RICORS, RD24/0004/0030},
publisher = {MDPI},
keywords = {biomarker},
keywords = {hydroxyproline},
keywords = {kidney},
title = {Hydroxyproline in Urine Microvesicles as a Biomarker of Fibrosis in the Renal Transplant Patient},
doi = {10.3390/biomedicines12122836},
author = {Torres Sanchez, Maria Jose and Ruiz Fuentes, María del carmen and Clavero García, Elena and Rísquez Chica, Noelia and Espinoza Muñoz, Karla and Espigares Huete, María José and Caba Molina, Mercedes and Osuna Ortega, Antonio and Wangensteen, Rosemary},
}