@misc{10481/98342,
year = {2024},
month = {12},
url = {https://hdl.handle.net/10481/98342},
abstract = {Background: Colorectal cancer (CRC) is the third diagnosed cancer worldwide. Forty-four
percent of metastatic colorectal cancer patients were diagnosed at an early stage. Despite curative
resection, approximately 40% of patients will develop metastases within a few years. Previous studies
indicate the presence of cancer stem cells (CSCs) and their contribution to CRC progression and
metastasis. miRNAs deregulation plays a role in CSCs formation and in tumor development. In
light of previous studies, we investigated the role of miR-486-5p to understand its role in CSC better.
Methods: The expression of miR-486-5p was assessed in adherent cells and spheres generated from
two CRC cell lines to observe the difference in expression in CSC-enriched spheroids. Afterward,
we overexpressed and underexpressed this miRNA in adherent and sphere cultures through the
transfection of a miR-486-5p mimic and a mimic inhibitor. Results: The results demonstrated that
miR-486-5p exhibited a notable downregulation in CSC models, and its overexpression led to a
significant decrease in colony size. Conclusions: In this study, we confirmed that miR-486-5p plays
an oncosuppressive role in CRC, thereby advancing our understanding of the role of this microRNA
in the CSC phenotype.},
organization = {MCIN/AEI/10.13039/501100011033/FEDER “Una manera
de hacer Europa”: RTI2018.101309B-C22},
organization = {The Chair “Doctors Galera-Requena in cancer
stem cell research” (CMC-CTS963)},
organization = {Junta de Andalucía, Plan Andaluz de Investigación,
Desarrollo e Innovación (grant POSTDOC_21_638 to C.G.-L.)},
publisher = {MDPI},
keywords = {colorectal cancer},
keywords = {spheres},
keywords = {cancer stem cells},
title = {The Role of miR-486-5p on CSCs Phenotypes in Colorectal Cancer},
doi = {10.3390/cancers16244237},
author = {Etzi, Federica and Griñán Lisón, Carmen and Fenu, Grazia and González Titos, Aitor and Pisano, Andrea and Farace, Cristiano and Sabalic, Angela and Picon-Ruiz, Manuel and Marchal Corrales, Juan Antonio and Madeddu, Roberto Beniamino Gublielmo},
}