@misc{10481/97955, year = {2019}, month = {11}, url = {https://hdl.handle.net/10481/97955}, abstract = {A simple method for direct detection of microRNAs (miRs) in human serum without the use of polymerase amplification is presented, achieving low miR-122 concentrations and importantly, discerning effectively single-base sequence mutations. The method is based on the capture of target miRs with synthetic peptide nucleic acid oligomers, dynamic chemical labelling, separation with quaternary amine microplatforms and detection using time-gated fluorescence imaging.}, publisher = {Royal Society of Chemistry}, title = {miR-122 direct detection in human serum by time-gated fluorescence imaging}, doi = {10.1039/C9CC08069D}, author = {García Fernández, Emilio and González García, María del Carmen and Pernagallo, Salvatore and Ruedas-Rama, Maria Jose and Fara, Mario Antonio and López Delgado, Francisco Javier and W. Dear, James and Ilyine, Hugh and Ress, Cristina and Díaz Mochón, Juan José and Orte Gutiérrez, Ángel}, }