@misc{10481/97905,
year = {2021},
month = {8},
url = {https://hdl.handle.net/10481/97905},
abstract = {Objectives
High cardiorespiratory fitness (CRF) levels reduce the risk of developing cardiovascular disease (CVD) during adulthood. However, little is known about the molecular mechanisms underlying the health benefits of high CRF levels at the early stage of life. This study aimed to analyze the whole-blood transcriptome profile of fit children with overweight/obesity (OW/OB) compared to unfit children with OW/OB.

Design
27 children with OW/OB (10.14 ± 1.3 years, 59% boys) from the ActiveBrains project were evaluated. VO2peak was assessed using a gas analyzer, and participants were categorized into fit or unfit according to the CVD risk-related cut-points. Whole-blood transcriptome profile (RNA sequencing) was analyzed. Differential gene expression analysis was performed using the limma R/Bioconductor software package (analyses adjusted by sex and maturational status), and pathways’ enrichment analysis was performed with DAVID. In addition, in silico validation data mining was performed using the PHENOPEDIA database.

Results
256 genes were differentially expressed in fit children with OW/OB compared to unfit children with OW/OB after adjusting by sex and maturational status (FDR < 0.05). Enriched pathway analysis identified gene pathways related to inflammation (eg, dopaminergic and GABAergic synapse pathways). Interestingly, in silico validation data mining detected a set of the differentially expressed genes to be related to CVD, metabolic syndrome, hypertension, inflammation, and asthma.

Conclusion
The distinct pattern of whole-blood gene expression in fit children with OW/OB reveals genes and gene pathways that might play a role in reducing CVD risk factors later in life.},
organization = {Spanish Ministry of Economy and Competitiveness, Grant/Award Number: DEP2013-47540, DEP2016-79512-R and DEP2017-91544- EXP},
organization = {European Regional Development Fund (FEDER), Grant/Award Number: RYC-2016-21199 and ENDORE SAF2017-87526-R},
organization = {Junta de Andalucía, Grant/Award Number: BIO-302 and US-1254251},
organization = {University of Jaén, Grant/ Award Number: PAIUJA-EI_CTS02},
organization = {Spanish Ministry of Education, Culture and Sport, Grant/Award Number: FPU 16/02760},
organization = {NIH, Grant/Award Number:UO1 TR002004},
organization = {PERC Systems Biology Fund; Unit of Excellence on EXERNET Research Network on Exercise and Health in Special Populations},
organization = {Alicia Koplowitz Foundation},
organization = {Henning Och Johan Throne-Holsts Stiftelse Grant},
organization = {University of Granada, Plan Propio de Investigación 2016, Excellence actions: Units of Excellence},
organization = {Unit of Excellence on Exercise and Health (UCEES)},
organization = {Junta de Andalucía, Consejería de Conocimiento, Investigación y Universidades and European Regional Development Fund (ERDF), Grant/ Award Number: SOMM17/6107/UGR},
organization = {European Regional Development Funds, Grant/Award Number: B-CTS-355-UGR18 and B-CTS-500-UGR18},
organization = {Universidad de Granada / CBUA},
publisher = {Wiley},
keywords = {aerobic fitness},
keywords = {childhood},
keywords = {gene expression},
title = {Cardiorespiratory fitness in children with overweight/ obesity: Insights into the molecular mechanisms},
doi = {10.1111/sms.14028},
author = {Plaza Florido, Abel Adrián and Altmäe, Signe and Esteban, Francisco J. and Löf, Marie and Radom-Aizik, Shlomit and Ortega Porcel, Francisco Bartolomé},
}