@misc{10481/97471,
year = {2024},
month = {11},
url = {https://hdl.handle.net/10481/97471},
abstract = {Liquid phase electron microscopy (LP-EM) has emerged as a powerful technique
for in situ observation of material formation in liquid. Especially the use
of graphene as window material provides new opportunities to image biological
processes because of graphene’s molecular thickness and electron scavenger
capabilities. However, in most cases the process of interest is initiated when
the graphene liquid cells (GLCs) are sealed, meaning that the process cannot
be imaged at early timepoints. Here, a novel cryogenic/liquid phase correlative
light/electron microscopy workflow that addresses the delay time between
graphene encapsulation and the start of the imaging, while combining the
advantages of fluorescence and electron microscopy is reported. This workflow
allows imaging to be initiated at a predetermined space and time by vitrifying
and thawing at a selected time point. The workflow is demonstrated first by
observing multiple day crystallization processes and subsequently highlight its
potential by observing a biological process: the complexation of calciprotein
particles. The ability to correlate the dynamic complexation observed in
a GLC with cryogenic TEM and dynamic light scattering, confirms the validity
of observations and underlines the exciting possibilities for LP-EM in biology.},
organization = {European Research
Council (ERC) Advanced Investigator grant (H2020-ERC-2017-
ADV-788982-COLMIN)},
organization = {Marie Curie Individual
Fellowship, MSCA-IF-2020 DYNAMIN (101031624)},
organization = {Research Program
Juan de la Cierva Incorporación (IJC2020-043639-I) funded by
MCIN/AEI/10.13039/501100011033},
organization = {European Union NextGenerationEU/
PRTR},
organization = {Project PID2022-141993NA-I00 funded by MICIU/
AEI/10.13039/501100011033 and ERDF/UE},
organization = {Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)
– TRR 219 – Project-ID 322900939, and 403041552},
publisher = {Wiley Online Library},
title = {A Cryo-to-Liquid Phase Correlative Light Electron Microscopy Workflow for the Visualization of Biological Processes in Graphene Liquid Cells},
doi = {10.1002/adfm.202416938},
author = {Rutten, Luco and Joosten, Ben and Schaart, Judith and de Beer, Marit and Roverts, Rona and Gräber, Steffen and Jahnen-Dechent, Willi and Akiva, Anat and Macías Sánchez, Elena and Sommerdijk, Nico},
}