@misc{10481/96052,
year = {2016},
month = {5},
url = {https://hdl.handle.net/10481/96052},
abstract = {The cis-acting replication element (CRE) of the hepatitis C virus (HCV) RNA genome is a region of
conserved sequence and structure at the 3′ end of the open reading frame. It participates in a complex
and dynamic RNA-RNA interaction network involving, among others, essential functional domains
of the 3′ untranslated region and the internal ribosome entry site located at the 5′ terminus of the
viral genome. A proper balance between all these contacts is critical for the control of viral replication
and translation, and is likely dependent on host factors. Proteomic analyses identified a collection
of proteins from a hepatoma cell line as CRE-interacting candidates. A large fraction of these were
RNA-binding proteins sharing highly conserved RNA recognition motifs. The vast majority of these
proteins were validated by bioinformatics tools that consider RNA-protein secondary structure.
Further characterization of representative proteins indicated that hnRNPA1 and HMGB1 exerted
negative effects on viral replication in a subgenomic HCV replication system. Furthermore DDX5 and
PARP1 knockdown reduced the HCV IRES activity, suggesting an involvement of these proteins in HCV
translation. The identification of all these host factors provides new clues regarding the function of the
CRE during viral cycle progression.},
organization = {Spanish Ministerio de Economía
y Competitividad (BFU2012-31213)},
organization = {Junta de Andalucía (CVI-7430)},
organization = {Junta de
Andalucía (CVI-7430)},
organization = {FEDER funds from the EU},
publisher = {Springer Nature},
title = {The cis-acting replication element of the Hepatitis C virus genome recruits host factors that influence viral replication and translation},
doi = {10.1038/srep25729},
author = {Ríos Marco, Pablo and Romero López, Cristina and Berzal Herranz, Alfredo},
}