@misc{10481/95688,
year = {2024},
month = {6},
url = {https://hdl.handle.net/10481/95688},
abstract = {Head and neck squamous cell carcinoma (HNSCC) is a highly malignant disease, and death rates have remained at approximately
50% for decades. New tumor-targeting strategies are desperately needed, and a previous report indicated the triggered
differentiation of HPV-negative HNSCC cells to confer therapeutic benefits. Using patient-derived tumor cells, we created a similar
HNSCC differentiation model of HPV+ tumor cells from two patients. We observed a loss of malignant characteristics in
differentiating cell culture conditions, including irregularly enlarged cell morphology, cell cycle arrest with downregulation of Ki67,
and reduced cell viability. RNA-Seq showed myocyte-like differentiation with upregulation of markers of myofibril assembly.
Immunofluorescence staining of differentiated and undifferentiated primary HPV+ HNSCC cells confirmed an upregulation of these
markers and the formation of parallel actin fibers reminiscent of myoblast-lineage cells. Moreover, immunofluorescence of HPV+
tumor tissue revealed areas of cells co-expressing the identified markers of myofibril assembly, HPV surrogate marker p16, and
stress-associated basal keratinocyte marker KRT17, indicating that the observed myocyte-like in vitro differentiation occurs in
human tissue. We are the first to report that carcinoma cells can undergo a triggered myocyte-like differentiation, and our study
suggests that the targeted differentiation of HPV+ HNSCCs might be therapeutically valuable.},
organization = {3-year Head and neck cancer program of the Medical
Faculty OWL at Bielefeld University},
organization = {Ministerio de Ciencia e Innovación/AEI: Agencia
Estatal de Investigación/10.13039/501100011033/y financiado por la Unión Europea
“NextGenerationEU”/PRTR (PID2020‐115112RB‐I00)},
organization = {Open Access funding enabled
and organized by Projekt DEAL},
publisher = {SpringerLink},
title = {Human papillomavirus-associated head and neck squamous cell carcinoma cells lose viability during triggered myocyte lineage differentiation},
doi = {10.1038/s41419-024-06867-4},
author = {Gendreizig, Sarah and Martínez Ruiz, Laura and López Rodríguez, Alba and Pabla, Harkiren and Hose, Leonie and Brasch, Frank and Busche, Tobias and Escames Rosa, Germaine and Sudhoff, Holger and Uwe Scholtz, Lars and Todt, Ingo and Oppel, Felix},
}