@misc{10481/95660,
year = {2015},
month = {4},
url = {https://hdl.handle.net/10481/95660},
abstract = {Four different types of polymeric particles with different functional groups on their surface have
been evaluated to develop biosensors using glucose oxidase as a model enzyme. Direct covalent
immobilization was achieved on particles functionalized with chloride, epoxy and vinyl groups via the
reactive functional groups on the surface, whereas particles functionalized with carboxylic groups, used
as reference materials, were pre-activated with carbodiimide. Immobilization was successfully performed
under very mild conditions (20  C, pH 8.0). In order to determine the advantages and disadvantages of
each functional group, both the amounts of immobilized enzymes and their relative activities were fully
investigated. In order to demonstrate their applicability on the design on biosensing with oxygen optical
transduction, the functionalized particles were co-immobilized in gold chips with oxygen sensing
particles (PSMA–PtTFPP) using electrophoretic deposition and characterised for glucose determination in
solution.},
organization = {Spanish Ministry of Economy and Competitiveness (CTQ2011-
25316, Ramon-Marquez's grant reference AP2012-0944 and
Medina-Castillo's Torres Quevedo contract reference PTQ-11-
04904)},
organization = {People Programme (Marie Curie Actions, Multi-
ITN) of the European Union’s Seventh Framework Programme
(project EUROMBR grant agreement no. 608104)},
publisher = {Royal Society of Chemistry},
title = {Evaluation of different functional groups for covalent immobilization of enzymes in the development of biosensors with oxygen optical transduction},
doi = {10.1039/C5AY00103J},
author = {Ramón Márquez, María Teresa and Medina Castillo, Antonio Luis and Fernández Sánchez, Jorge Fernando and Fernández Gutiérrez, Alberto},
}