@misc{10481/95638,
year = {2016},
month = {11},
url = {https://hdl.handle.net/10481/95638},
abstract = {Aims
to design calcium and zinc-loaded bioactive and cytocompatible nanoparticles for the treatment
of periodontal disease.
Methods
PolymP-nActive nanoparticles were zinc or calcium loaded. Biomimetic calcium phosphate
precipitation on polymeric particles was assessed after 7 days immersion in simulated body
fluid, by scanning electron microscopy attached to an energy dispersive analysis system.
Amorphous mineral deposition was probed by X-ray diffraction. Cell viability analysis was
performed using oral mucosa fibroblasts by: 1) quantifying the liberated deoxyribonucleic
acid from dead cells, 2) detecting the amount of lactate dehydrogenase enzyme released
by cells with damaged membranes, and 3) by examining the cytoplasmic esterase function
and cell membranes integrity with a fluorescence-based method using the Live/Dead commercial
kit. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests.
Results
Precipitation of calcium and phosphate on the nanoparticles surfaces was observed in calcium-loaded nanoparticles. Non-loaded nanoparticles were found to be non-toxic in all the
assays, calcium and zinc-loaded particles presented a dose dependent but very low cytotoxic
effect.
Conclusions
The ability of calcium-loaded nanoparticles to promote precipitation of calcium phosphate
deposits, together with their observed non-toxicity may offer new strategies for periodontal
disease treatment.},
organization = {Ministry
of Economy and Competitiveness and European
Regional Development Fund, Grant: MAT2014-52036-P},
publisher = {Plos One},
title = {Bioactive Polymeric Nanoparticles for Periodontal Therapy},
doi = {10.1371/journal.pone.0166217},
author = {Osorio Ruiz, Raquel and Alfonso-Rodríguez, Camilo Andrés and Medina Castillo, Antonio Luis and Alaminos Mingorance, Miguel and Toledano Pérez, Manuel},
}