@misc{10481/95551,
year = {2024},
month = {9},
url = {https://hdl.handle.net/10481/95551},
abstract = {Head and neck squamous cell carcinoma (HNSCC) presents a significant challenge worldwide due to its
aggressiveness and high recurrence rates post-treatment, often linked to cancer stem cells (CSCs). Melatonin
shows promise as a potent tumor suppressor; however, the effects of melatonin on CSCs remain unclear, and the
development of models that closely resemble tumor heterogeneity could help to better understand the effects of
this molecule. This study developed a tumor scaffold based on patient fibroblast-derived decellularized extracellular
matrix that mimics the HNSCC microenvironment. Our study investigates the antitumoral effects of
melatonin within this context. We validated its strong antiproliferative effect on HNSCC CSCs and the reduction
of tumor invasion and migration markers, even in a strongly chemoprotective environment, as it is required to
increase the minimum doses necessary to impact tumor viability compared to the non-scaffolded tumorspheres
culture. Moreover, melatonin exhibited no cytotoxic effects on healthy cells co-cultured in the tumor hydrogel.
This scaffold-based platform allows an in vitro study closer to HNSCC tumor reality, including CSCs, stromal
component, and a biomimetic matrix, providing a new valuable research tool in precision oncology.},
organization = {Plan Propio of University of Granada (PPJIB2020.3)},
organization = {MCIN/AEI/10.13039/501100011033/ (PID2020-115112RB-I00)},
organization = {European Union NextGenerationEU/ PRTR (PID2022-140151OB-C22)},
organization = {Chair “Doctors Galera- Requena in cancer stem cell research” (CMC-CTS963)},
publisher = {Elsevier},
keywords = {Head and neck squamous cell carcinoma},
keywords = {Cancer stem cells},
keywords = {Melatonin},
title = {A bioengineered tumor matrix-based scaffold for the evaluation of melatonin efficacy on head and neck squamous cancer stem cells},
doi = {10.1016/j.mtbio.2024.101246},
author = {López de Andrés, Julia and Rodríguez Santana, César and Lara-Peña, Laura de and Jiménez González, Gema and Escames Rosa, Germaine and Marchal Corrales, Juan Antonio},
}