@misc{10481/95421,
year = {2023},
month = {5},
url = {https://hdl.handle.net/10481/95421},
abstract = {Cryo-correlative light and electron microscopy (cryoCLEM) is a powerful strategy to high
resolution imaging in the unperturbed hydrated state. In this approach fluorescence microscopy
aids localizing the area of interest, and cryogenic focused ion beam/scanning electron
microscopy (cryoFIB/SEM) allows preparation of thin cryo-lamellae for cryoET. However, the
current method cannot be accurately applied on bulky (3D) samples such as tissues and
organoids. 3D cryo-correlative imaging of large volumes is needed to close the resolution gap
between cryo-light microscopy and cryoET, placing sub-nanometer observations in a larger
biological context. Currently technological hurdles render 3D cryoCLEM an unexplored
approach. Here we demonstrate a cryoCLEM workflow for tissues, correlating cryo-Airyscan
confocal microscopy with 3D cryoFIB/SEM volume imaging. Accurate correlation is achieved
by imprinting a FinderTOP pattern in the sample surface during high pressure freezing, and
allows precise targeting for cryoFIB/SEM volume imaging.},
organization = {European Research Council (ERC) Advanced
Investigator grant (H2020-ERC-2017-ADV-788982-COLMIN)},
organization = {VENI grant from the Netherlands Scientific Organization NWO
(VI.Veni.192.094)},
organization = {Marie Skłodowska Curie Individual Fellowship (H2020-MSCA-IF-2020- 101031624- DYNAMIN)},
publisher = {Springer Nature},
title = {Precise targeting for 3D cryo-correlative light and electron microscopy volume imaging of tissues using a FinderTOP},
doi = {10.1038/s42003-023-04887-y},
author = {de Beer, Marit and Daviran, Deniz and Roverts, Rona and Rutten, Luco and Macías Sánchez, Elena and R. Metz, Juriaan and Sommerdijk, Nico and Akiva, Anat},
}