@misc{10481/94954,
year = {2022},
url = {https://hdl.handle.net/10481/94954},
abstract = {Coexistence of different populations of cells and isolation of tasks can provide
enhanced robustness and adaptability or impart new functionalities to a culture. However,
generating stable cocultures involving cells with vastly different growth rates can be challenging.
To address this, we developed living analytics in a multilayer polymer shell (LAMPS), an
encapsulation method that facilitates the coculture of mammalian and bacterial cells. We leverage
LAMPS to preprogram a separation of tasks within the coculture: growth and therapeutic protein
production by the mammalian cells and L -lactate biosensing by Escherichia coli encapsulated within
LAMPS. LAMPS enable the formation of a synthetic bacterial−mammalian cell interaction that
enables a living biosensor to be integrated into a biomanufacturing process. Our work serves as a
proof-of-concept for further applications in bioprocessing since LAMPS combine the simplicity
and flexibility of a bacterial biosensor with a viable method to prevent runaway growth that would
disturb mammalian cell physiology.},
organization = {EPSRC Adventurous Manufacturing program (EP/T005297/1)},
organization = {EPSRC Frontiers Engineering program (EP/K038648/1)},
organization = {BBSRC (BB/S006206/1)},
organization = {Spanish Ministry of Science and Innovation  IJC2019-041817-I/AIE/10.13039/ 501100011033},
publisher = {ACS},
keywords = {Coculture},
keywords = {Biosensor},
keywords = {Bacteria},
keywords = {Mammalian cells},
keywords = {L-lactate},
keywords = {Hydrogel encapsulation},
title = {Polymer encapsulation of bacterial biosensors enables coculture with mammalian cells},
doi = {10.1021/acssynbio.1c00577},
author = {Moya-Ramírez, Ignacio and Kotidis, Pavlos and Marbiah, Masue and Kim, Juhyun and Kontoravdi, Cleo and Polizzi, Karen},
}