@misc{10481/94870,
year = {2006},
month = {4},
url = {https://hdl.handle.net/10481/94870},
abstract = {The endothelial cell monolayer of cerebral vessels and its basement membrane (BM) are
ensheathed by the astrocyte endfeet, the leptomeningeal cells, and their associated parenchymal BM, all of which contribute to establishment of the blood–brain barrier (BBB). As a
consequence of this unique structure, leukocyte penetration of cerebral vessels is a multistep event. In mouse experimental autoimmune encephalomyelitis (EAE), a widely used
central nervous system infl ammatory model, leukocytes fi rst penetrate the endothelial cell
monolayer and underlying BM using integrin 𝛃 1-mediated processes, but mechanisms used
to penetrate the second barrier defi ned by the parenchymal BM and glia limitans remain
uninvestigated. We show here that macrophage-derived gelatinase (matrix metalloproteinase
[MMP]-2 and MMP-9) activity is crucial for leukocyte penetration of the parenchymal BM.
Dystroglycan, a transmembrane receptor that anchors astrocyte endfeet to the parenchymal
BM via high affi nity interactions with laminins 1 and 2, perlecan and agrin, is identifi ed as
a specifi c substrate of MMP-2 and MMP-9. Ablation of both MMP-2 and MMP-9 in
double knockout mice confers resistance to EAE by inhibiting dystroglycan cleavage and
preventing leukocyte infi ltration. This is the fi rst description of selective in situ proteolytic
damage of a BBB-specifi c molecule at sites of leukocyte infi ltration.},
organization = {German and Swedish Research Councils (grant nos. So285/5-1, So285/5-2); (grant nos. K2005-06X-14184-04A, 621-2001- 2142)},
organization = {Alfred Österlunds, Knut and Alice Wallenbergs (grant no. 2002.0056)},
organization = {Greta and Johan Kocks Foundations},
organization = {Fund for Scientifi c Research-Flanders},
organization = {“Geconcerteerde OnderzoeksActies”},
organization = {Charcot Foundation},
publisher = {The Rockefeller University Press},
keywords = {Dystroglycan},
keywords = {Parenchymal basement membrane},
keywords = {Leukocyte extravasation},
keywords = {Autoimmune encephalomyelitis},
title = {Dystroglycan is selectively cleaved at the parenchymal basement membrane at sites of leukocyte extravasation in experimental autoimmune encephalomyelitis},
doi = {10.1084/jem.20051342},
author = {Agrawal, Smriti and Anderson, Per and Durbeej, Madeleine and Van Rooijen, Nico and Ivars, Fredrik and Opdenakker, Ghislain and Sorokin, Lydia M.},
}