@misc{10481/94350, year = {2024}, month = {8}, url = {https://hdl.handle.net/10481/94350}, abstract = {This study explored the cultivation of Porphyridium cruentum using beet molasses and corn steep liquor (CSL) as alternative agri-waste substrates. The objective was to enhance the biomass and lipid production of P. cruentum, known for its potential as an industrial EPA producer. Using a Box-Behnken Design (BBD) as part of the response surface methodology (RSM), we investigated the effects of beet molasses, CSL, and F/2 nutrients on the microalga's cultivation. The results demonstrated a significant influence of these factors on the final cell count and lipid content over an 8-day cultivation period. Specifically, optimal growth conditions were identified at approximately 1.78 g/L of molasses and 1.89 g/L of CSL, yielding a cell count of 12.1 × 106 cells/mL and a lipid content of 24.48%. Validation experiments reaffirmed these findings, with observed results closely aligning with predicted values. Optimized media achieved also a significant higher content of carotenoids and phycobiliproteins compared to the standard media. This research highlights the potential of using agro-industrial by-products for large-scale cultivation of P. cruentum, offering a sustainable approach for enhancing lipid and pigment production.}, organization = {ERA-Net SUSFOOD2 project SUSPUFA, ID 145, with funding provided by the Italian Ministry of Education, Universities, and Research (MIUR) and the Spanish Ministry of Science, Education, and Universities/Spanish State Research Agency (PCI 2018-093,178)}, organization = {European Union's Horizon 2020 research and innovation program}, publisher = {Elsevier}, keywords = {Food waste}, keywords = {Microalgae}, keywords = {Waste valorization}, title = {Sustainable cultivation of Porphyridium cruentum via agroindustrial by-products: A study on biomass and lipid enhancement}, doi = {10.1016/j.bcab.2024.103341}, author = {Luca Russo, Giovanni and Luca Langellotti, Antonio and Verardo, Vito and Martín García, Beatriz and Oliviero, Maria and Masi, Paolo}, }