@misc{10481/94004,
year = {2021},
month = {6},
url = {https://hdl.handle.net/10481/94004},
abstract = {Objectives: To analyze how novel developed silicon dioxide composite membranes, functionalized with zinc or
doxycycline, can modulate the expression of genes related to the osteogenic functional capacity of osteoblastic
cells.
Methods: The composite nanofibers membranes were manufactured by using a novel polymeric blend and 20 nm
silicon dioxide nanoparticles (SiO2-NPs). To manufacture the membranes, 20 nm SiO2-NPs were added to the
polymer solution and the resulting suspension was processed by electrospinning. In a second step, the membranes were functionalized with zinc or doxycycline. Then, they were subjected to MG63 osteoblast-like cells
culturing for 48 h. After this time, real-time quantitative polymerase chain reaction (RT-qPCR) was carried out to
study the expression of Runx-2, OSX, ALP, OSC, OPG, RANKL, Col-I, BMP-2, BMP-7, TGF-β1, VEGF, TGF-βR1,
TGF- βR2, and TGF-βR3. Mean comparisons were conducted by One-way ANOVA and Tukey tests (p < 0.05).
Results: In general, the blending of SiO2-NPs in the tested non-resorbable polymeric scaffold improves the
expression of osteogenic genes over the control membranes. Doxycycline doping of experimental scaffolds
attained the best results, encountering up-regulation of BMP-2, ALP, OPG, TGFβ-1 and TGFβ-R1. Membranes
with zinc induced a significant increase in the expression of Col-I, ALP and TGF β1. Both, zinc and doxycycline
functionalized membranes enormously down-regulated the expression of RANKL.
Conclusions: Zinc and doxycycline doped membranes are bioactive inducing overexpression of several osteogenic
gene markers.
Clinical significance: Doxycycline doped membranes may be a potential candidate for use in GBR procedures in
several challenging pathologies, including periodontal diseases.},
organization = {Ministry of Economy and Competitiveness-European Regional Development Fund[Project MAT2017-85999-P MINECO/AEI/FEDER/UE]},
organization = {University of Granada/Regional Government of Andalusia (A-BIO-157-UGR-18/FEDER)},
publisher = {Elsevier},
keywords = {RT-qPCR},
keywords = {Doxycycline},
keywords = {Gene expression},
keywords = {GBR},
keywords = {Membrane},
title = {Doxycycline-doped membranes induced osteogenic gene expression on osteoblastic cells},
doi = {10.1016/j.jdent.2021.103676},
author = {Toledano Osorio, Manuel and Manzano-Moreno, Francisco Javier and Toledano Pérez, Manuel and Osorio Ruiz, Raquel and Medina Castillo, Antonio Luis and Costela Ruiz, Víctor Javier and Ruiz Rodríguez, Concepción},
}