@misc{10481/93831,
year = {2024},
month = {7},
url = {https://hdl.handle.net/10481/93831},
abstract = {Objectives To evaluate the effect of proanthocyanidin-functionalized hydroxyapatite nanoparticles (nHAp_PA) used as pretreatment at different concentrations on the microtensile bond strength (µTBS) and endogenous enzymatic activity (MMPs) on pH-cycled dentin after 24 h and 6 months of artificial aging. Materials and methods Fifty human sound dentin blocks were randomly assigned to 5 groups (n = 10): (i) negative control (no treatment); (ii) positive control (pH-cycling); (iii) pH-cycling + 2% nHAp_PA for 60s; (iv) pH-cycling + 6.5% nHAp_PA for 60s; (v) pH-cycling + 15% nHAp_PA for 60s. A self-etch adhesive was used for bonding procedures before resin composite build-ups. Specimens were tested with the µTBS test after 24 h and 6 months of laboratory storage. The proteolytic activity in each group was evaluated with gelatin zymography and in situ zymography. Data were statistically analyzed (p < 0.05). Results At 24 h, the µTBS of the experimental groups were significantly higher than the controls (p ≤ 0.001), and no differences were observed between different concentrations (p > 0.05). Artificial aging significantly decreased bond strength in all groups (p ≤ 0.008); however, nHAp_PA 2% still yielded higher bonding values than controls (p ≤ 0.007). The groups pretreated with nHAp_PA exhibited lower MMP-9 and MMP-2 activities compared to the positive control group and almost the same enzymatic activity as the negative control group. In situ zymography showed that after 6 months of aging, nHAp_PA 2% and nHAp_PA 6,5% decreased enzymatic activity as well as the negative control. Conclusions Dentin pretreatment with nHAp_PA increased the bonding performance of a self-etch adhesive and decreased MMP-2 and MMP-9 activities after 6 months.},
organization = {Funding for open access charge: Universidad de Granada / CBUA},
publisher = {Springer},
keywords = {Collagen cross-linkers},
keywords = {Dentin biomodification},
keywords = {Nanomaterials},
keywords = {MMPs},
keywords = {Zymography},
keywords = {Microtensile bond strength},
title = {Effects of proanthocyanidin-functionalized hydroxyapatite nanoparticles on dentin bonding},
doi = {https://doi.org/10.1007/s00784-024-05836-7},
author = {Enrich Essvein, Tattiana and González López, Santiago and Rodríguez Navarro, Alejandro and Cifuentes Jiménez, Carolina Cecilia and Maravic, Tatjana and Mazzitelli, Claudia and Checchi, Vittorio and Josic, Uros and Mazzoni, Annalisa and Breschi, Lorenzo},
}