@misc{10481/93799,
year = {2024},
month = {7},
url = {https://hdl.handle.net/10481/93799},
abstract = {Y chromosome short tandem repeats (Y-STRs) typing is a useful tool in scenarios such as mass graves analysis or
disaster victim identification and has become a routine analysis in many laboratories. Not many comparisons
have been performed with the currently available commercial kits, much less with degraded skeletal remains.
This research aims to evaluate the performance of three commercial Y-STR kits: Yfiler™ Plus, PowerPlex® Y23,
and Investigator® Argus Y-28 in 63 degraded skeletal remains from mass graves. PowerPlex® Y23 yields more
reportable markers and twice the RFU on average, while Yfiler™ Plus and Investigator® Argus Y-28 exhibited a
similar behaviour. Additionally, Argus Y-28, which has not been tested with this kind of samples in literature
before, showed a good performance. Finally, a predictive model was attempted to be developed from quantification
and autosomal STR data. However, no acceptable model could be obtained. Nevertheless, good Y-STR
typing results may be expected if at least 50 pg DNA input is used or 13 autosomal markers were previously
obtained.},
organization = {Spanish Ministry of Universities for funding
the development of his PhD (FPU 20/01967)},
organization = {Universidad de Granada / CBUA},
publisher = {Elsevier},
keywords = {Degraded human DNA},
keywords = {Degraded samples},
keywords = {Skeletal remains},
title = {Evaluating the efficacy of three Y-STRs commercial kits in degraded skeletal remains},
doi = {10.1016/j.scijus.2024.07.007},
author = {Haarkötter Cardoso, Christian and Medina Lozano, María Isabel and Vinueza Espinosa, Diana C. and Sáiz Guinaldo, María and Gálvez Escolano, Xiomara and Álvarez Merino, Juan Carlos and Lorente Acosta, José Antonio},
}