@misc{10481/93681,
year = {2024},
month = {4},
url = {https://hdl.handle.net/10481/93681},
abstract = {Three-dimensional (3D) bioprinting is considered one of the most advanced tools to build up materials for tissue engineering.
The aim of this work was the design, development and characterization of a bioink composed of human mesenchymal
stromal cells (hMSC) for extrusion through nozzles to create these 3D structures that might potentially be apply to replace
the function of damaged natural tissue. In this study, we focused on the advantages and the wide potential of biocompatible
biomaterials, such as hyaluronic acid and alginate for the inclusion of hMSC. The bioink was characterized for its physical
(pH, osmolality, degradation, swelling, porosity, surface electrical properties, conductivity, and surface structure), mechanical
(rheology and printability) and biological (viability and proliferation) properties. The developed bioink showed high
porosity and high swelling capacity, while the degradation rate was dependent on the temperature. The bioink also showed
negative electrical surface and appropriate rheological properties required for bioprinting. Moreover, stress-stability studies
did not show any sign of physical instability. The developed bioink provided an excellent environment for the promotion of
the viability and growth of hMSC cells. Our work reports the first-time study of the effect of storage temperature on the cell
viability of bioinks, besides showing that our bioink promoted a high cell viability after being extruded by the bioprinter.
These results support the suggestion that the developed hMSC-composed bioink fulfills all the requirements for tissue engineering
and can be proposed as a biological tool with potential applications in regenerative medicine and tissue engineering.},
organization = {Ministry of Economy and Competitiveness
(FEDER funds), grant number RTC-2016-5451-1},
organization = {Ministry of
Economy and Competitiveness, Instituto de Salud Carlos III (FEDER
funds), grant numbers DTS19/00143 and DTS17/00087)},
organization = {Consejería
de Economía, Conocimiento, Empresas y Universidad de la Junta de
Andalucía, grant numbers P18-FR-2470, PYC20 RE 015 UGR, A-CTS-
180-UGR20 and B-CTS-230-UGR18},
organization = {Fundación Mutua Madrileña,
grant number FMM-AP17196-2019},
organization = {AdvanceCat with the support of Acció (Catalonia
Trade & Investment; Generalitat de Catalunya) under the Catalonian
European Regional Development Fund operational program,
2014–2020},
organization = {Fundação para a Ciência e a
Tecnologia, I.P., Lisbon, Portugal.},
publisher = {Springer},
keywords = {Human mesenchymal stromal cells},
keywords = {Bioink},
keywords = {Regenerative medicine},
title = {Human mesenchymal stromal cells-laden crosslinked hyaluronic acidalginate bioink for 3D bioprinting applications in tissue engineering},
doi = {10.1007/s13346-024-01596-9},
author = {Galocha-León, Cristina and Antich, Cristina and Voltes Martínez, Ana and Marchal, Juan A and Mallandrich, Mireia and Halbaut, Lyda and B. Souto, Elena and Gálvez Martín, Patricia and Clares Naveros, Beatriz},
}