@misc{10481/93613,
year = {2024},
month = {5},
url = {https://hdl.handle.net/10481/93613},
abstract = {It is known that selenium (Se) is an essential trace element, important for the growth
and other biological functions of fish. One of its most important functions is to contribute to
the preservation of certain biological components, such as DNA, proteins, and lipids, providing
protection against free radicals resulting from normal metabolism. The objective of this study was to
evaluate and optimize selenium accumulation in the native yeast Rhodotorula mucilaginosa 6S. Sodium
selenite was evaluated at different concentrations (5–10–15–20–30–40 mg/L). Similarly, the effects
of different concentrations of nitrogen sources and pH on cell growth and selenium accumulation
in the yeast were analyzed. Subsequently, the best cultivation conditions were scaled up to a 2 L
reactor with constant aeration, and the proteome of the yeast cultured with and without sodium
selenite was evaluated. The optimal conditions for biomass generation and selenium accumulation
were found with ammonium chloride and pH 5.5. Incorporating sodium selenite (30 mg/L) during
the exponential phase in the bioreactor after 72 h of cultivation resulted in 10 g/L of biomass, with
0.25 mg total Se/g biomass, composed of 25% proteins, 15% lipids, and 0.850 mg total carotenoids/g
biomass. The analysis of the proteomes associated with yeast cultivation with and without selenium
revealed a total of 1871 proteins. The results obtained showed that the dynamic changes in the
proteome, in response to selenium in the experimental medium, are directly related to catalytic
activity and oxidoreductase activity in the yeast. R. mucilaginosa 6S could be an alternative for the
generation of selenium-rich biomass with a composition of other nutritional compounds also of
interest in aquaculture, such as proteins, lipids, and pigments.},
organization = {ANID/Fondecyt postdoctoral project 3210499},
publisher = {MDPI},
keywords = {Rhodotorula mucilaginosa},
keywords = {Selenium-enriched yeasts},
keywords = {Selenium},
title = {Enhancing Selenium Accumulation in Rhodotorula mucilaginosa Strain 6S Using a Proteomic Approach for Aquafeed Development},
doi = {10.3390/biom14060629},
author = {Díaz-Navarrete, Paola and Sáez-Arteaga, Alberto and Marileo, Luis and Alors, David and Correa Galeote, David and Dantagnan, Patricio},
}