@misc{10481/92703,
year = {2024},
month = {2},
url = {https://hdl.handle.net/10481/92703},
abstract = {Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis,
a rapid and acute infection of the central nervous system with a fatal outcome
in >97% of cases. Due to the infrequent report of cases and diagnostic gaps that
hinder the possibility of recovering clinic isolates, studies related to pathogenesis
of the disease are scarce. However, the secretion of cytolytic molecules has
been proposed as a factor involved in the progression of the infection. Several
of these molecules could be included in extracellular vesicles (EVs), making
them potential virulence factors and even modulators of the immune response
in this infection. In this work, we evaluated the immunomodulatory effect of
EVs secreted by two clinic isolates of Naegleria fowleri using in vitro models.
For this purpose, characterization analyses between EVs produced by both
isolates were first performed, for subsequent gene transcription analyses post
incubation of these vesicles with primary cultures from mouse cell microglia
and BV-2 cells. Analyses of morphological changes induced in primary culture
microglia cells by the vesicles were also included, as well as the determination
of the presence of nucleic acids of N. fowleri in the EV fractions. Results revealed
increased expression of NOS, proinflammatory cytokines IL-6, TNF-α, and IL-23,
and the regulatory cytokine IL-10 in primary cultures of microglia, as well as
increased expression of NOS and IL-13 in BV-2 cells. Morphologic changes from
homeostatic microglia, with small cellular body and long processes to a more
amoeboid morphology were also observed after the incubation of these cells
with EVs. Regarding the presence of nucleic acids, specific Naegleria fowleri
DNA that could be amplified using both conventional and qPCR was confirmed
in the EV fractions. Altogether, these results confirm the immunomodulatory
effects of EVs of Naegleria fowleri over microglial cells and suggest a potential
role of these vesicles as biomarkers of primary acute meningoencephalitis.},
organization = {Projects C-2600: “Secreción de vesículas extracelulares por Naegleria fowleri y evaluación de su potencial rol inmunomodulador en un modelo in vitro” and C-1061: “Caracterización de antígenos de excreción/secreción y antígenos somáticos en amebas de vida libre mediante el empleo de anticuerpos policlonales producidos en roedores”, supported by Vicerrectoría de Investigación of the University of Costa Rica},
organization = {Oficina de Asuntos Internacionales y Cooperación Externa (OAICE) of Universidad de Costa Rica},
organization = {Fundación Carolina (Spain)},
organization = {“Contrato de Formación de Profesorado Universitario” of the Ministry of Science, Culture and Sports (Spain)},
publisher = {Frontiers Media},
keywords = {Extracellular vesicles},
keywords = {Trophozoites},
keywords = {Naegleria fowleri},
title = {Providing an in vitro depiction of microglial cells challenged with immunostimulatory extracellular vesicles of Naegleria fowleri},
doi = {10.3389/fmicb.2024.1346021},
author = {Retana Moreira, Lissette and Cornet Gómez, Alberto and Sepúlveda Justo, María Del Rosario and Molina Castro, Silvia and Alvarado Ocampo, Johan and Chaves Monge, Frida and Jara Rojas, Mariana and Osuna Carrillo De Albornoz, Antonio and Abrahams Sandí, Elizabeth},
}