@misc{10481/92557,
year = {2024},
month = {1},
url = {https://hdl.handle.net/10481/92557},
abstract = {Every year million tons of by-products and waste from olive and orange processing are produced by agri-food
industries, thus triggering environmental and economic problems worldwide. From the perspective of a circular
economy model, olive leaves and orange peels can be valorized in valuable products due to the presence of
bioactive compounds such as polyphenols exhibiting beneficial effects on human health.
The aqueous extracts of olive leaves and orange peels rich in phenolic compounds were prepared by
ultrasound-assisted extraction. Both extracts were characterized in terms of yield of extraction, total phenolic
content and antioxidant capacity; the polyphenolic profiles were deeper investigated by HPLC-MS analysis.
Each extract was included in liposomes composed by a natural phospholipid, 1,2-dioleoyl-sn-glycero-3-phosphocholine, and cholesterol prepared according to the thin-layer evaporation method coupled with a sonication
process.
The antimicrobial activity of the extracts, free and loaded in liposomes, was investigated according to the
broth macrodilution method against different strains of potential bacterial pathogenic species: Staphylococcus
aureus (NCIMB 9518), Bacillus subtilis (ATCC 6051) and Enterococcus faecalis (NCIMB 775) as Gram-positive,
while Escherichia coli (NCIMB 13302), Pseudomonas aeruginosa (NCIMB 9904) and Klebsiella oxytoca (NCIMB
12259) as Gram-negative.
The encapsulation of olive leaves extract in liposomes enhanced its antibacterial activity against S. aureus by
an order of magnitude.},
organization = {SHEALTHY project (grant number 817936)},
organization = {CNR project NUTR-AGE (FOE-2021 DBA.AD005.225)},
organization = {“One Health Basic and Translational Research Actions addressing Unmet Needs on Emerging Infectious Diseases - IN-FACT” - PNRR Next Generation EU project (project number
PE00000007)},
organization = {PRIN 2017 project entitled Bachounds: Supramolecular
nanostructures for bacteria detection},
publisher = {Elsevier},
keywords = {Ultrasound assisted extraction},
keywords = {Olive leaves extract},
keywords = {Orange peels extract},
title = {Ultrasound assisted extraction and liposome encapsulation of olive leaves and orange peels: How to transform biomass waste into valuable resources with antimicrobial activity},
doi = {10.1016/j.ultsonch.2024.106765},
author = {Prevete, Giuliana and Carvalho, Loïc G. and Razola Díaz, María del Carmen and Verardo, Vito and Mancini, Giovanna and Fiore, Alberto and Mazzonna, Marco},
}