@misc{10481/92515,
year = {2024},
month = {1},
url = {https://hdl.handle.net/10481/92515},
abstract = {Complex mixtures of disinfection by-products (DBPs) are present in disinfected waters, but their mixture toxicity
has been rarely described. Apart from ingestion, DBP exposure can occur through inhalation, which may lead to
respiratory effects in highly exposed individuals. However, the underlying biological mechanisms have yet to be
elucidated. This study aimed to investigate the toxicity of a mixture of 10 DBPs, including haloacetic acids and
haloaromatics, on human alveolar A549 cells by assessing their cytotoxicity, genotoxicity, and impact on the cell
lipidome. A DBP mixture up to 50 μM slightly reduced cell viability, induced the generation of reactive oxygen
species (ROS) up to 3.5-fold, and increased the frequency of micronuclei formation. Exposure to 50 μM DBP
mixture led to a significant accumulation of triacylglycerides and a decrease of diacylglycerides and phosphatidylcholines
in A549 cells. Lipidomic profiling of extracellular vesicles (EVs) released in the culture medium
revealed a marked increase in cholesterol esters, sphingomyelins, and other membrane lipids. Overall, these
alterations in the lipidome of cells and EVs may indicate a disruption of lipid homeostasis, and thus, potentially
contribute to the respiratory effects associated with DBP exposure.},
organization = {Projects PID2021-122592NB-I00 and
CEX2018-000794-S (Ministerio de Ciencia e Innovación)},
organization = {ComFuturo Programme (2nd edition) funded by the
Fundación General del CSIC},
organization = {Grant RYC2020-028901-I funded by MCIN/AEI/10.13039/501100011033 and “ESF investing in your
future”},
publisher = {Elsevier},
keywords = {A549 cell line},
keywords = {Mixture toxicity},
keywords = {Haloacids},
title = {Toxicity and underlying lipidomic alterations generated by a mixture of water disinfection byproducts in human lung cells},
doi = {10.1016/j.scitotenv.2024.170331},
author = {Hosseinzadeh, Mahboubeh and Postigo, Cristina and Porte, Cinta},
}