@misc{10481/92482,
year = {2024},
month = {8},
url = {https://hdl.handle.net/10481/92482},
abstract = {Pantoea agglomerans is considered one of the most ubiquitous and versatile organisms that include strains that
induce diseases in various crops and occasionally cause opportunistic infections in humans. To develop effective
strategies to mitigate its impact on plant health and agricultural productivity, a comprehensive investigation is
crucial for better understanding its pathogenicity. One proposed eco-friendly approach involves the enzymatic
degradation of quorum sensing (QS) signal molecules like N-acylhomoserine lactones (AHLs), known as quorum
quenching (QQ), offering potential treatment for such bacterial diseases. In this study the production of C4 and 3-
oxo-C6HSL was identified in the plant pathogenic P. agglomerans CFBP 11141 and correlated to enzymatic activities
such as amylase and acid phosphatase. Moreover, the heterologous expression of a QQ enzyme in the
pathogen resulted in lack of AHLs production and the attenuation of the virulence by mean of drastically
reduction of soft rot disease in carrots and cherry tomatoes. Additionally, the interference with the QS systems of
P. agglomerans CFBP 11141 by two the plant growth-promoting and AHL-degrading bacteria (PGP-QQ) Pseudomonas
segetis P6 and Bacillus toyonensis AA1EC1 was evaluated as a potential biocontrol approach for the first
time. P. segetis P6 and B. toyonensis AA1EC1 demonstrated effectiveness in diminishing soft rot symptoms induced
by P. agglomerans CFBP 11141 in both carrots and cherry tomatoes. Furthermore, the virulence of pathogen
notably decreased when co-cultured with strain AA1EC1 on tomato plants.},
organization = {Spanish Ministry of the Economy and Competitiveness (PID2019-106704RB-100/AEI/10.13039/501100011033)},
organization = {Junta de Andalucía  B-AGR-222-UGR20},
organization = {ERDF},
organization = {Universidad de Granada / CBUA},
publisher = {Elsevier},
keywords = {Plant growth-promoting bacteria},
keywords = {Quorum quenching},
keywords = {Plant pathogens},
keywords = {Quorum sensing},
title = {Interference of AHL signal production in the phytophatogen Pantoea agglomerans as a sustainable biological strategy to reduce its virulence},
doi = {10.1016/j.micres.2024.127781},
author = {Amaro-da Cruz, Alba and Cabeo Garrido, Mónica and Durán-Viseras, Ana and Sampedro Quesada, María Inmaculada and Llamas Company, Inmaculada},
}