@misc{10481/91595,
year = {2015},
month = {8},
url = {https://hdl.handle.net/10481/91595},
abstract = {Objectives: Characterization of a urine isolate of daptomycin non-susceptible Enterococcus faecium recovered from a patient with kidney transplantation and no history of daptomycin exposure.

Methods: After isolation in a urine sample, identification of E. faecium was confirmed by amplification of the E. faecium-specific gene encoding D-alanyl-D-alanine ligase (ddl) and daptomycin susceptibility testing was performed by E-test on cation-adjusted Mueller-Hinton agar. In order to determine the genetic bases of daptomycin resistance, the open reading frames of five genes previously associated with daptomycin resistance in enterococci were sequenced.

Results: Substitutions in the response regulator LiaR (S19F) and cardiolipin synthase (R218Q) were identified.

Conclusions: To the best of our knowledge, this is the first characterization of emerging daptomycin resistance in E. faecium in a Spanish hospital in the absence of daptomycin exposure and in a renal transplant recipient.},
abstract = {Objetivos. Presentamos la caracterización de un aislado
de Enterococcus faecium no sensible a daptomicina, recuperado de una muestra de orina de un paciente con trasplante de riñón e infección urinaria y sin antecedentes de exposición
previa a daptomicina.
Métodos. Tras el aislamiento, la identificación de E. faecium fue confirmada por la amplificación del gen que codifica
la región específica de la ligasa de la D-alanil-D-alanina (ddl)
y la prueba de sensibilidad a daptomicina se realizó mediante
E-test en agar Mueller-Hinton ajustado para cationes. Con el
fin de determinar las bases genéticas de la resistencia a daptomicina, se secuenciaron las regiones de lectura abierta de cinco
genes previamente asociados con la resistencia a daptomicina
en enterococos.
Resultados. Se identificaron cambios en el promotor de
LiaR (S19F) y la sintetasa de la cardiolipina (R218Q).
Conclusiones. Esta es la primera caracterización de un
aislado clínico de E. faecium con resistencia a daptomicina en
un hospital español, en ausencia de exposición previa y en un
receptor de trasplante renal.},
organization = {National Institutes of Health
[NIH]  R01 AI093749},
publisher = {Sociedad Española de Quimioterapia},
keywords = {Enterococcus faecium},
keywords = {Daptomycin non-susceptible},
keywords = {LiaR},
keywords = {Cardiolipin synthase},
keywords = {Daptomicina},
keywords = {Cardiolipina sintetasa},
title = {Characterization of daptomycin non-susceptible Enterococcus faecium producing urinary tract infection in a renal transplant recipient},
author = {Sorlozano Puerto, Antonio and Panesso, Diana and Navarro-Marí, José María and Arias, César A. and Gutiérrez Fernández, José},
}