@misc{10481/91472,
year = {2021},
month = {10},
url = {https://hdl.handle.net/10481/91472},
abstract = {Objective: To characterize the Staphylococcus aureus strains colonizing healthy Spanish children.

Methods: Between March and July 2018, 1876 Spanish children younger than 14 years attending primary healthcare centers were recruited from rural and urban areas. Staphylococcus aureus colonization of the anterior nostrils was analyzed. MecA and mecC genes, antibiotic susceptibility, and genotyping according to the spa were determined in all strains, and the following toxins were examined: Panton-Valentine leucocidin (pvl), toxic shock syndrome toxin (tst), and exfoliative toxins (eta, etb, etd). Multilocus sequence typing (MLST) and staphylococcal cassette chromosome (SCCmec) typing were performed on methicillin-resistant Staphylococcus aureus (MRSA) strains, as well as pulsed-field gel electrophoresis (PFGE).

Results: 619 strains were isolated in 1876 children (33%), and 92% of them were sent for characterization to the Spanish National Centre of Microbiology (n = 572). Twenty (3.5%) of these strains were mecA-positive. Several spa types were detected among MRSA, being t002 the most frequently observed (30%), associating with SCCmec IVc. Among MSSA, 33% were positive for tst, while only 0.73% were positive for pvl. The 20 MRSA strains were negative for pvl, and 6 (30%) harbored the tst gene.

Conclusions: methicillin-resistant Staphylococcus aureus nasal colonization in Spanish children is rare, with t002 being the most observed spa type, associated with SCCmec IVc. None of the MRSA strains produced pvl, but up to 30% of S. aureus strains were positive for tst.},
publisher = {Microbiologyopen .},
title = {Characterization of methicillin-resistant Staphylococcus aureus strains colonizing the nostrils of Spanish children},
doi = {10.1002/mbo3.1235},
author = {Román, Federico and Mendez-Echevarria, Ana and Del Rosal, Teresa and Garcia-Vera, Cesar and Escosa-Garcia, Luis and Agud, Martin and Chaves, Fernando and Gutiérrez Fernández, José and Ruiz de Gopegui, Enrique and Ruiz-Carrascoso, Guillermo and Ruiz-Gallego, Maria Del Carmen and Bernet, Albert and Maria Quevedo, Sara and Fernández-Verdugo, Ana Maria and Sainz, Talia and Calvo, Cristina},
}