@misc{10481/91254,
year = {2024},
month = {1},
url = {https://hdl.handle.net/10481/91254},
abstract = {Pseudomonas aeruginosa is one of the most common microorganisms causing infections
of severe skin wounds. Antibiotic or antiseptic treatments are crucial to prevent and curb these
infections. Antiseptics have been reported to be cytotoxic to skin cells and few studies evaluate
the impact of commonly used antibiotics. This study evaluates how clinical antibiotics affect skin
cells’ viability, proliferation, migration, and cytokine secretion and defines the highest non-cytotoxic
concentrations that maintain antibacterial activity. Cell proliferation, viability, and migration were
evaluated on cell monolayers. Cytokines related to the wound healing process were determined.
The minimum inhibitory concentrations and the impact on bacterial biofilm were assessed. Results
showed that 0.02 mg/mL ciprofloxacin and 1 mg/mL meropenem are the highest non-cytotoxic
concentrations for fibroblasts and keratinocytes while 1.25 mg/mL amikacin and 0.034 mg/mL
colistin do not affect fibroblasts’ viability and cytokine secretion but have an impact on keratinocytes.
These concentrations are above the minimum inhibitory concentration but only amikacin could
eradicate the biofilm. For the other antibiotics, cytotoxic concentrations are needed to eradicate the
biofilm. Combinations with colistin at non-cytotoxic concentrations effectively eliminate the biofilm.
These results provide information about the concentrations required when administering topical
antibiotic treatments on skin lesions, and how these antibiotics affect wound management therapies.
This study set the basis for the development of novel antibacterial wound healing strategies such as
antibiotic artificial skin substitutes.},
organization = {Predoctoral fellowship (FPU19/05455, BOE
22 October 2019) from the Ministry of Science, Innovation and Universities of Spain},
organization = {Ministry of Health and
Families of the Andalusian Regional Government (PIGE-0242-2019)},
organization = {Carlos III Health Institute
(PI17/02083)},
organization = {General Program of the European Molecular Biology Organization (EMBO
Scientific Exchange Grant 10007)},
organization = {Swedish
Research Council (2022-01202)},
organization = {Region Stockholm (FoUI-961229)},
organization = {Center for Innovative
Medicine (FoUI-975603)},
publisher = {MDPI},
keywords = {Antibiotics},
keywords = {Biofilm},
keywords = {Fibroblasts},
title = {Antibiotics against Pseudomonas aeruginosa on Human Skin Cell Lines: Determination of the Highest Non-Cytotoxic Concentrations with Antibiofilm Capacity for Wound Healing Strategies},
doi = {10.3390/pharmaceutics16010117},
author = {Quiñones Vico, María Isabel and Fernández González, Ana and Ubago Rodríguez, Ana Dolores and Moll, Kirsten and Norrby-Teglund, Anna and Svensson, Mattias and Gutiérrez Fernández, José and Torres De Pinedo, Jesús Manuel and Arias Santiago, Salvador Antonio},
}