@misc{10481/91115,
year = {2024},
month = {1},
url = {https://hdl.handle.net/10481/91115},
abstract = {Asthma is a multifactorial condition that can be associated with obesity. The phenotypes of asthma in lean and obese patients are different, with proinflammatory signatures being further elevated in the latter. Both obesity and asthma are associated with alterations in intestinal barrier function and immunity, and with the composition of the intestinal microbiota and food consumption. In this study, we aimed to establish an organoid model to test the hypothesis that the intestinal content of lean and obese, allergic, asthmatic children differentially regulates epithelial intestinal gene expression. A model of mouse jejunum intestinal organoids was used. A group of healthy, normal-weight children was used as a control. The intestinal content of asthmatic obese children differentially induced the expression of inflammatory and mitochondrial response genes (Tnf-tumor necrosis factor, Cd14, Muc13-mucin 13, Tff2-Trefoil factor 2 and Tff3, Cldn1-claudin 1 and 5, Reg3g-regenerating family member 3 gamma, mt-Nd1-NADH dehydrogenase 1 and 6, and mt-Cyb-mitochondrial cytochrome b) via the RAGE-advanced glycosylation end product-specific receptor, NF-κB-nuclear factor kappa b and AKT kinase signal transduction pathways. Fecal homogenates from asthmatic normal-weight and obese children induce a differential phenotype in intestinal organoids, in which the presence of obesity plays a major role.},
organization = {Fundacion Progreso y Salud Project number PI-0373-2014},
organization = {Agencia Andaluza del Conocimiento, Regional Government of Andalucía and European Regional
Development Fund through project A-CTS-263-UGR18},
organization = {Grant
PID2020-112768RB-I00 funded by MICINN},
organization = {Grant from Fondo de Investigaciones
Sanitarias, Instituto de Salud Carlos III, Spain (PI21/00952)},
organization = {Grants A-AGR-468-
UGR20 and P20-00695 funded by Junta de Andalucía and FEDER},
organization = {The Spanish Government through an FPU grant},
organization = {Instituto de Salud Carlos III, Spain},
publisher = {MDPI},
keywords = {Organoids},
keywords = {Fecal content},
keywords = {Gastrointestinal microbiome},
title = {Differential Modulation of Mouse Intestinal Organoids with Fecal Luminal Factors from Obese, Allergic, Asthmatic Children},
doi = {10.3390/ijms25020866},
author = {Córdova, Samir and Tena Garitaonaindia, Mireia and Álvarez Mercado, Ana Isabel and Gámez Belmonte, María de los Reyes and Gómez Llorente, Mª Amelia and Sánchez De Medina López-Huertas, Fermín and Martínez Cañavate, Ana and Martínez Augustín, María Olga and Gómez Llorente, Carolina},
}