@misc{10481/91111,
year = {2024},
month = {1},
url = {https://hdl.handle.net/10481/91111},
abstract = {The chorioallantoic membrane (CAM) model, generated during avian development, can
be used in cancer research as an alternative in vivo model to perform tumorigenesis in ovo due
to advantages such as simplicity, low cost, rapid growth, and being naturally immunodeficient.
The aim of this systematic review has been to compile and analyze all studies that use the CAM
assay as a tumor induction model. For that, a systematic search was carried out in four different
databases: PubMed, Scopus, Cochrane, and WOS. After eliminating duplicates and following the
established inclusion and exclusion criteria, a total of 74 articles were included. Of these, 62% use
the in ovo technique, 13% use the ex ovo technique, 9% study the formation of metastasis, and 16%
induce tumors from patient biopsies. Regarding the methodology followed, the main species used is
chicken (95%), although some studies use quail eggs (4%), and one article uses ostrich eggs. Therefore,
the CAM assay is a revolutionary technique that allows a simple and effective way to induce tumors,
test the effectiveness of treatments, carry out metastasis studies, perform biopsy grafts of patients,
and carry out personalized medicine. However, unification of the methodology used is necessary.},
organization = {Instituto de Salud Carlos III (PI19/ 01478-FEDER)},
organization = {Project PMPTA22/00136 funded by the Instituto de Salud Carlos III-FEDER},
organization = {CPP2022-009967 and CPP2022-010017 Project from the Spanish Ministry of
Science and Innovation (FEDER)},
publisher = {MDPI},
keywords = {CAM},
keywords = {Tumor},
keywords = {Xenograft},
title = {Experimental Tumor Induction and Evaluation of Its Treatment in the Chicken Embryo Chorioallantoic Membrane Model: A Systematic Review},
doi = {10.3390/ijms25020837},
author = {Mesas Hernández, Cristina and Chico Lozano, María Ángeles and Doello, Kevin and Lara, Patricia and Moreno Pérez, Javier and Melguizo Alonso, Consolación and Perazzoli, Gloria and Prados Salazar, José Carlos},
}