@misc{10481/91047, year = {2023}, month = {11}, url = {https://hdl.handle.net/10481/91047}, abstract = {Gelatin nanoparticles (GNPs) have been widely studied for a plethora of biomedical applications, but their formation mechanism remains poorly understood, which precludes precise control over their physicochemical properties. This leads to timeconsuming parameter adjustments without a fundamental grasp of the underlying mechanism. Here, we analyze and visualize in a timeresolved manner the mechanism by which GNPs are formed during desolvation of gelatin as a function of gelatin molecular weight and type of desolvating agent. Through various analytical and imaging techniques, we unveil a multistage process that is initiated by the formation of primary particles that are ∼18 nm in diameter (wet state). These primary particles subsequently assemble into colloidally stable GNPs with a raspberry-like structure and a hydrodynamic diameter of ∼300 nm. Our results create a basic understanding of the formation mechanism of gelatin nanoparticles, which opens new opportunities for precisely tuning their physicochemical and biofunctional properties.}, organization = {Radboud University Medical Center}, publisher = {American Chemical Society}, keywords = {Gelatin nanoparticles}, keywords = {Desolvation}, keywords = {Self-assembly}, title = {Unraveling the Formation of Gelatin Nanospheres by Means of Desolvation}, doi = {10.1021/acs.nanolett.3c03459}, author = {Besheli, Negar Hassani and Martens, Martijn and Macías Sánchez, Elena and Olijve, Jos and Yang, Fang and Sommerdijk, Nico and Leeuwenburgh, Sander C. G.}, }